EMPIAR-11689 - Single-particle Cryo-EM datasets of hydroxycarboxylic acid receptor signaling complexes [multiple data sets in TIFF format]

EMPIAR-11689

Single-particle Cryo-EM datasets of hydroxycarboxylic acid receptor signaling complexes [multiple data sets in TIFF format]

Publication:

Structural basis of hydroxycarboxylic acid receptor signaling mechanisms through ligand binding

Suzuki S, Tanaka K, Nishikawa K, Suzuki H, Oshima A, Fujiyoshi Y

Nature communications 14 (2023)

PMID: 37736747

DOI: 10.1038/s41467-023-41650-7

Related EMDB entry:

EMD-35442 EMD-35445 EMD-35444 EMD-35446 EMD-35443

Deposited:

2023-09-14

Released:

2023-10-03

Last modified:

2023-10-03

Imageset size:

5.10 TB

Imageset DOI:

10.6019/EMPIAR-11689

Experimental metadata:

Download xml json

Deposited via PDBj

Contains:

micrographs - multiframe

Image Sets

1. Unaligned multi-frame TIFF compressed movies of the HCA2-Gi complex with GSK256073

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

4872

Image size:

(5760, 4092)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(0.83, 0.83)

Details:

Please discard the first frame, which was corrupted due to a problem with the CDS mode.
Gain reference (MRC format) is included in each folder with the movie files. No rotation or flipping is required in RELION.
The pixel size in the TIFF header is not correct.
HT: 300 kV, Cs: 2.7 mm. does per frame: 1 e/Å2.

Files:

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2. Unaligned multi-frame TIFF compressed movies of the HCA2-Gi complex with MK6892

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

5806

Image size:

(5760, 4092)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(0.83, 0.83)

Details:

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

3. Unaligned multi-frame TIFF compressed movies of the HCA2-Gi complex with LUF6283

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

2828

Image size:

(3710, 3838)

Pixel type:

UNSIGNED 16 BIT INTEGER

Pixel spacing:

(0.99, 0.99)

Details:

Gain reference (MRC format) is included in each folder with the movie files. No rotation or flipping is required in RELION.
The number at the end of the file indicates the direction of the 3x3 image shift.
The pixel size in the TIFF header is not correct.
HT: 300 kV, Cs: 3.4 mm. does per frame: 1.74 e/Å2

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

4. Unaligned multi-frame TIFF compressed movies of the HCA2-Gi complex with acifran

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

3602

Image size:

(3710, 3838)

Pixel type:

UNSIGNED 16 BIT INTEGER

Pixel spacing:

(0.99, 0.99)

Details:

Gain reference (MRC format) is included in each folder with the movie files.
The number at the end of the file indicates the direction of the 3x3 image shift.
The pixel size in the TIFF header is not correct.
HT: 300 kV, Cs: 3.4 mm. does per frame: 1.74 e/Å2

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

5. Unaligned multi-frame TIFF compressed movies of the HCA3-Gi complex with acifran

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

6440

Image size:

(3710, 3838)

Pixel type:

UNSIGNED 16 BIT INTEGER

Pixel spacing:

(0.78, 0.78)

Details:

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

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PERC: a suite of software tools for the curation of cryoEM data with application to simulation, modeling and machine learning.

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