EMPIAR-11685 - Structural basis of peptidoglycan synthesis by E. coli RodA-PBP2 complex [11120 multi-frame micrographs composed of 50 frames each in TIFF format]

EMPIAR-11685

Structural basis of peptidoglycan synthesis by E. coli RodA-PBP2 complex [11120 multi-frame micrographs composed of 50 frames each in TIFF format]

Publication:

Structural basis of peptidoglycan synthesis by E. coli RodA-PBP2 complex

Nygaard R, Graham CLB, Belcher Dufrisne M, Colburn JD, Pepe J, Hydorn MA, Corradi S, Brown CM, Ashraf KU, Vickery ON, Briggs NS, Deering JJ, Kloss B, Botta B, Clarke OB, Columbus L, Dworkin J, Stansfeld PJ, Roper DI, Mancia F

Nature communications 14 (2023)

PMID: 37620344

DOI: 10.1038/s41467-023-40483-8

Related EMDB entry:

EMD-41299 EMD-41304 EMD-41303

Deposited:

2023-07-20

Released:

2023-09-25

Last modified:

2023-09-25

Imageset size:

2.76 TB

Imageset DOI:

10.6019/EMPIAR-11685

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe

Image Sets

1. Unaligned 50 frame micrographs of RodA-PBP2 in nanodisc

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

11120

Image size:

(5760, 4092)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(0.83, 0.83)

Details:

The micrographs are of 50 raw movie frames accumulating 58.5 electrons per squared Angstrom in a 2.5 second exposure. The data was collected on a 300 kV Krios and the spherical abberation is 2.7 mm.

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Citations

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Schäfer JH, Calza A, Hom K, Damodar P, Peng R, Bogdanović N, Lander GC, Stagg SM, Cianfrocco MA. (2025)