EMPIAR-11685 - Structural basis of peptidoglycan synthesis by E. coli RodA-PBP2 complex [11120 multi-frame micrographs composed of 50 frames each in TIFF format]

EMPIAR-11685

Structural basis of peptidoglycan synthesis by E. coli RodA-PBP2 complex [11120 multi-frame micrographs composed of 50 frames each in TIFF format]

Publication:

Structural basis of peptidoglycan synthesis by E. coli RodA-PBP2 complex

Nygaard R, Graham CLB, Belcher Dufrisne M, Colburn JD, Pepe J, Hydorn MA, Corradi S, Brown CM, Ashraf KU, Vickery ON, Briggs NS, Deering JJ, Kloss B, Botta B, Clarke OB, Columbus L, Dworkin J, Stansfeld PJ, Roper DI, Mancia F

Nature communications 14 (2023)

PMID: 37620344

DOI: 10.1038/s41467-023-40483-8

Related EMDB entry:

EMD-41299 EMD-41304 EMD-41303

Deposited:

2023-07-20

Released:

2023-09-25

Last modified:

2023-09-25

Imageset size:

2.76 TB

Imageset DOI:

10.6019/EMPIAR-11685

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe

Image Sets

1. Unaligned 50 frame micrographs of RodA-PBP2 in nanodisc

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

11120

Image size:

(5760, 4092)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(0.83, 0.83)

Details:

The micrographs are of 50 raw movie frames accumulating 58.5 electrons per squared Angstrom in a 2.5 second exposure. The data was collected on a 300 kV Krios and the spherical abberation is 2.7 mm.

Files:

This entry cannot be downloaded from PDBj.

Archives and downloads the selected files into an uncompressed zip file.

Depending on the number and size of files to be downloaded, this can take an enormous amount of time. Also, this feature is unstable and may not work properly, and checksums of downloaded files are not verified.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

Browse All Files

Quick Links

Talks and Tutorials

EMPIAR Quick tour

Statistics

Publications

Re-use case study

EMPIAR in the news

Claim entries to your ORCiD

COVID-19 Data Portal

EMDB

PDBe

BioImage Archive

EMNavigator@PDBj

PDBj

RCSB@PDBj

EMDataResoruce@RCSB,EMDB,NCMI

Citations

Small LEA proteins mitigate air-water interface damage to fragile cryo-EM samples during plunge freezing.

Abe KM, Li G, He Q, Grant T, Lim CJ. (2024)

Outer membrane protein assembly mediated by BAM-SurA complexes.

Fenn KL, Horne JE, Crossley JA, Böhringer N, Horne RJ, Schäberle TF, Calabrese AN, Radford SE, Ranson NA. (2024)

Ubiquitinated histone H2B as gatekeeper of the nucleosome acidic patch.

Hicks CW, Rahman S, Gloor SL, Fields JK, Husby NL, Vaidya A, Maier KE, Morgan M, Keogh MC, Wolberger C. (2024)

Molecular recognition of an odorant by the murine trace amine-associated receptor TAAR7f.

Gusach A, Lee Y, Khoshgrudi AN, Mukhaleva E, Ma N, Koers EJ, Chen Q, Edwards PC, Huang F, Kim J, Mancia F, Veprintsev DB, Vaidehi N, Weyand SN, Tate CG. (2024)

The novel ribosome biogenesis inhibitor usnic acid blocks nucleolar pre-60S maturation.

Kofler L, Grundmann L, Gerhalter M, Prattes M, Merl-Pham J, Zisser G, Grishkovskaya I, Hodirnau VV, Vareka M, Breinbauer R, Hauck SM, Haselbach D, Bergler H. (2024)

See all citations

Protein Data Bank Japan News

[2024-11-28]

[wwPDB] Access SDF/MOL formatted CCD files from the PDB archive

[2024-11-27]

374 new PDB entries have been released on 2024-11-27.

[2024-11-01]

A new portal integrating genome variants and protein structures has released

[2024-10-30]

[wwPDB] Deprecation of FTP File Download Protocol on November 1, 2024

[2024-10-25]

PDBj luncheon seminar at APBJC 2024