EMPIAR-11638
Single particle cryo-EM dataset of Bos taurus mitochondrial complex I (initially solubilised in DDM) proteoliposomes [5538 multi-frame micrographs composed of 40 frames each in TIFF format]
Publication:

Molecular mechanism of the ischemia-induced regulatory switch in mammalian complex I

Grba DN, Wright JJ, Fisher W, Yin Z, Hirst J

Science (New York, N.Y.) 384 (2024) 1247-1253

PMID: 38870289

Deposited:
2023-07-26
Released:
2024-07-09
Last modified:
2024-07-09
Imageset size:
1.45 TB
Imageset DOI:
Experimental metadata:
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Contains:
  • micrographs - multiframe
1. Unaligned multi-frame micrographs of wild-type bos taurus mitochondrial complex I, solubilised in DDM, reconstituted into proteoliposomes.
Category:
micrographs - multiframe
Image format:
TIFF
No. of images or tilt series:
5538
Image size:
(5760, 4092)
Pixel type:
UNSIGNED BYTE
Pixel spacing:
(1.43, 1.43)
Details:
Unaligned multi-frame micrographs of wild-type bovine mitochondrial complex I from an as-prepared sample solubilised in DDM and reconstituted in to liposomes. Frozen on graphene oxide gold grids. Images (TIFF) were collected with a 300 keV FEI Titan Krios on a Gatan K3 detector in super-resolution mode but bin2 at the hardware stage, using counting mode and a Gatan BioContinuum energy filter (20 eV). The C2 and objective lens apertures were set to 70 and 100 µm, respectively. 3.4 s exposures were captured in 40 frames with a total dose of ~40 Å-2. A pixel size of 1.43 (calibrated 1.35) Å/pixel was used.
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