EMPIAR-11638 - Single particle cryo-EM dataset of Bos taurus mitochondrial complex I (initially solubilised in DDM) proteoliposomes [5538 multi-frame micrographs composed of 40 frames each in TIFF format]

EMPIAR-11638

Single particle cryo-EM dataset of Bos taurus mitochondrial complex I (initially solubilised in DDM) proteoliposomes [5538 multi-frame micrographs composed of 40 frames each in TIFF format]

Publication:

Molecular mechanism of the ischemia-induced regulatory switch in mammalian complex I

Grba DN, Wright JJ, Fisher W, Yin Z, Hirst J

Science (New York, N.Y.) 384 (2024) 1247-1253

PMID: 38870289

DOI: 10.1126/science.ado2075

Related EMDB entry:

EMD-18057 EMD-18051 EMD-18059 EMD-18055 EMD-18052 EMD-18054

Deposited:

2023-07-26

Released:

2024-07-09

Last modified:

2024-07-09

Imageset size:

1.45 TB

Imageset DOI:

10.6019/EMPIAR-11638

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe

Image Sets

1. Unaligned multi-frame micrographs of wild-type bos taurus mitochondrial complex I, solubilised in DDM, reconstituted into proteoliposomes.

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

5538

Image size:

(5760, 4092)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(1.43, 1.43)

Details:

Unaligned multi-frame micrographs of wild-type bovine mitochondrial complex I from an as-prepared sample solubilised in DDM and reconstituted in to liposomes. Frozen on graphene oxide gold grids. Images (TIFF) were collected with a 300 keV FEI Titan Krios on a Gatan K3 detector in super-resolution mode but bin2 at the hardware stage, using counting mode and a Gatan BioContinuum energy filter (20 eV). The C2 and objective lens apertures were set to 70 and 100 µm, respectively. 3.4 s exposures were captured in 40 frames with a total dose of ~40 Å-2. A pixel size of 1.43 (calibrated 1.35) Å/pixel was used.

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