EMPIAR-11636
Single particle cryo-EM dataset, collected with a 20 degree tilt, of Bos taurus mitochondrial complex I (originally purified in LMNG) proteoliposomes and deactivated [4550 multi-frame micrographs composed of 40 frames each in TIFF format]
Publication:

Molecular mechanism of the ischemia-induced regulatory switch in mammalian complex I

Grba DN, Wright JJ, Fisher W, Yin Z, Hirst J

Science (New York, N.Y.) 384 (2024) 1247-1253

PMID: 38870289

Related EMDB entry:
Deposited:
2023-08-01
Released:
2024-07-03
Last modified:
2024-07-03
Imageset size:
959.66 GB
Imageset DOI:
Experimental metadata:
Download xml json
Contains:
  • micrographs - multiframe
1. Unaligned multi-frame micrographs of wild-type bos taurus mitochondrial complex I, solubilised in LMNG, reconstituted into proteoliposomes and deactivated. Collected with 20 degree tilt.
Category:
micrographs - multiframe
Image format:
TIFF
No. of images or tilt series:
4550
Image size:
(5760, 4092)
Pixel type:
UNSIGNED BYTE
Pixel spacing:
(1.072, 1.072)
Details:
Unaligned multi-frame micrographs of wild-type bovine mitochondrial complex I from an as-prepared sample solubilised in LMNG and reconstituted in to liposomes. Not gain corrected (gain file included). The proteoliposomes were heat treated (37 ºC, 20 min) to deactivate the sample, before being frozen on graphene oxide gold grids. Images were collected with a 20º tilt. Images (TIFF) were collected with a 300 keV FEI Titan Krios on a Gatan K3 detector in super-resolution mode but bin2 at the hardware stage, using counting mode and a Gatan BioContinuum energy filter (20 eV). The C2 and objective lens apertures were set to 50 and 100 µm, respectively. 3.8 s exposures were captured in 40 frames with a total dose of ~40 Å-2. A pixel size of 1.072 Å/pixel was used.
Files:
Loading...
Files:
Loading...

Ito F, Alvarez-Cabrera AL, Liu S, Yang H, Shiriaeva A, Zhou ZH, Chen XS. (2023)
Rigden DJ, Fernández XM. (2023)
Iudin A, Korir PK, Somasundharam S, Weyand S, Cattavitello C, Fonseca N, Salih O, Kleywegt GJ, Patwardhan A. (2023)
Serra Lleti JM, Steyer AM, Schieber NL, Neumann B, Tischer C, Hilsenstein V, Holtstrom M, Unrau D, Kirmse R, Lucocq JM, Pepperkok R, Schwab Y. (2023)
Caldwell BJ, Norris AS, Karbowski CF, Wiegand AM, Wysocki VH, Bell CE. (2022)