EMPIAR-11618
Single-particle cryo-EM dataset of an asymmetric nucleosome (10-N-0) [multiple data sets in TIFF and MRCS formats]
Publication:

Single-particle cryo-EM dataset of an asymmetric nucleosome (10-N-0)

Gaullier G

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Related EMDB entry:
Deposited:
2023-07-05
Released:
2023-08-25
Last modified:
2023-08-25
Imageset size:
1.15 TB
Imageset DOI:
Experimental metadata:
Download xml json
Contains:
  • micrographs - multiframe
  • picked particles - single frame - processed
1. Unaligned movies
Category:
micrographs - multiframe
Image format:
TIFF
No. of images or tilt series:
1537
Image size:
(4096, 4096)
Pixel type:
SIGNED 16 BIT INTEGER
Pixel spacing:
(0.952, 0.952)
Details:
The pixel size of 0.9783 A/pix in the tiff header is wrong, the correct one is 0.952 as indicated in the deposition metadata. This discrepancy comes from the fact that this dataset was collected during commissioning of the microscope, before the pixel size at this magnification was calibrated.

The coordinates deposited here are the particles that gave the reconstruction deposited as EMD-17944.
Files:
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2. Extracted particles
Category:
picked particles - single frame - processed
Image format:
MRCS
No. of images or tilt series:
68401
Image size:
(256, 256)
Pixel type:
32 BIT FLOAT
Pixel spacing:
(0.952, 0.952)
Details:
Particle stack extracted from motion-corrected micrographs. Running ab initio reconstruction and 3D refinement on them should reproduce the reconstruction deposited as EMD-17944.
Files:
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Files:
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Ito F, Alvarez-Cabrera AL, Liu S, Yang H, Shiriaeva A, Zhou ZH, Chen XS. (2023)
Rigden DJ, Fernández XM. (2023)
Iudin A, Korir PK, Somasundharam S, Weyand S, Cattavitello C, Fonseca N, Salih O, Kleywegt GJ, Patwardhan A. (2023)
Serra Lleti JM, Steyer AM, Schieber NL, Neumann B, Tischer C, Hilsenstein V, Holtstrom M, Unrau D, Kirmse R, Lucocq JM, Pepperkok R, Schwab Y. (2023)
Caldwell BJ, Norris AS, Karbowski CF, Wiegand AM, Wysocki VH, Bell CE. (2022)