EMPIAR-11607 - Automethylated PRC2 dimer bound to nucleosome [multiple data sets in MRC format]

EMPIAR-11607

Automethylated PRC2 dimer bound to nucleosome [multiple data sets in MRC format]

Publication:

Activation of automethylated PRC2 by dimerization on chromatin

Sauer PV, Pavlenko E, Cookis T, Zirden LC, Renn J, Singhal A, Hunold P, Hoehne-Wiechmann MN, van Ray O, Kaschani F, Kaiser M, Hänsel-Hertsch R, Sanbonmatsu KY, Nogales E, Poepsel S

Molecular cell (2024)

PMID: 39303719

DOI: 10.1016/j.molcel.2024.08.025

Related EMDB entry:

EMD-41146 EMD-41110 EMD-41141

Deposited:

2023-06-28

Released:

2024-11-08

Last modified:

2024-11-08

Imageset size:

1.84 TB

Imageset DOI:

10.6019/EMPIAR-11607

Experimental metadata:

Download xml json

Contains:

micrographs - single frame

Image Sets

1. Dataset1, automethylated PRC2 dimer bound to nucleosome, motion corrected, dose weighted, streptavidin subtracted

Category:

micrographs - single frame

Image format:

MRC

No. of images or tilt series:

3894

Image size:

(3710, 3838)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(1.15, 1.15)

Details:

Motion corrected, dose weighted, streptavidin subtracted, dose 40 electron per square angstrom

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2. Dataset2, automethylated PRC2 dimer bound to nucleosome, motion corrected, dose weighted, streptavidin subtracted

Category:

micrographs - single frame

Image format:

MRC

No. of images or tilt series:

4062

Image size:

(3710, 3838)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(1.15, 1.15)

Details:

Motion corrected, dose weighted, streptavidin subtracted, dose 40 electron per square angstrom

Files:

This entry cannot be downloaded from PDBj.

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3. Dataset4, PRC2_J119-450 bound to H1-nucleosome, motion corrected, dose weighted, streptavidin subtracted

Category:

micrographs - single frame

Image format:

MRC

No. of images or tilt series:

14470

Image size:

(4092, 5760)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(0.94, 0.94)

Details:

Motion corrected, dose weighted, streptavidin subtracted, dose 50 electron per square angstrom

Files:

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4. Dataset3, H1 bound to nucleosome

Category:

micrographs - single frame

Image format:

MRC

No. of images or tilt series:

2236

Image size:

(4092, 5760)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(1.14, 1.14)

Details:

The sample used here was PRC2 (EZH2, SUZ12,EED, RBAP48, AEBP2, but without Jarid2) together with H1.0 bound to nucleosome. However it didn't form a complex. Instead, this dataset yielded a structure of H1.0 bound to nucleosome alone. In addition, the quality of the streptavidin lattice was not good, so the subtraction was incomplete. Therefore, when processing this dataset there might be leftovers of streptavidin appearing in the 2D classes. This dataset was recorded on a Talos Arctica at 200kV with a dose of 50 electrons per square Angstrom.

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