EMPIAR-11606
Cryo-EM structure NDUFS4 knockout complex I from Mus musculus kidney [3373 multi-frame micrographs composed of 59 frames each in MRC format]
Publication:

Structural insights into respiratory complex I deficiency and assembly from the mitochondrial disease-related ndufs4-/- mouse.

Yin Z, Agip ANA, Bridges HR, Hirst J

The EMBO journal (2024)

PMID: 38177503

Related EMDB entry:
Deposited:
2023-07-03
Released:
2024-02-01
Last modified:
2024-02-01
Imageset size:
3.79 TB
Imageset DOI:
Experimental metadata:
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Compressed by PDBj:

Image formats and pixel types shown below are those of the original, non-compressed files.

MRC movies (FoilHole*.mrc) in this entry were compressed by bzip2 (pbzip2)
by EMPIAR Japan to save space and bandwidth.

Some programs (e.g. RELION >= 4.0.1 and CryoSPARC) can process compressed movies without explicit
decompression. For other programs, movies have to be decompressed beforehand. In this case,
you might want to convert decompressed MRC movies into TIFF to save your local storage.

Original size:   6,521,075,216 kilobytes
Compressed size: 4,066,339,120 kilobytes
Contains:
  • micrographs - multiframe
1. Unaligned multi-frame micrographs of NDUFS4 knockout complex I from Mus musculus kidney
Category:
micrographs - multiframe
Image format:
MRC
No. of images or tilt series:
3373
Image size:
(4096, 4096)
Pixel type:
SIGNED 16 BIT INTEGER
Pixel spacing:
(1.46, 1.46)
Details:
Micrographs were imaged using a Falcon III detector in integrating mode, each with 59 movie frames using the FEI EPU software. The calibrated pixel size was 1.46 Å, and the total electron dose was 49.24 electrons Å-2 over a total exposure time of 1.5 s for each micrograph.
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Citations

Ito F, Alvarez-Cabrera AL, Liu S, Yang H, Shiriaeva A, Zhou ZH, Chen XS. (2023)
Rigden DJ, Fernández XM. (2023)
Iudin A, Korir PK, Somasundharam S, Weyand S, Cattavitello C, Fonseca N, Salih O, Kleywegt GJ, Patwardhan A. (2023)
Serra Lleti JM, Steyer AM, Schieber NL, Neumann B, Tischer C, Hilsenstein V, Holtstrom M, Unrau D, Kirmse R, Lucocq JM, Pepperkok R, Schwab Y. (2023)
Caldwell BJ, Norris AS, Karbowski CF, Wiegand AM, Wysocki VH, Bell CE. (2022)