EMPIAR-11596 - Seeded Aβ(1-40) amyloid fibril [3018 multi-frame micrographs composed of 40 frames each in TIFF format]

EMPIAR-11596

Seeded Aβ(1-40) amyloid fibril [3018 multi-frame micrographs composed of 40 frames each in TIFF format]

Publication:

Cryo-EM analysis of the effect of seeding with brain-derived Aβ amyloid fibrils

Pfeiffer PB, Ugrina M, Schwierz N, Sigurdson CJ, Schmidt M, Fändrich M

(---)

Related EMDB entry:

EMD-17168

Deposited:

2023-05-31

Released:

2024-03-18

Last modified:

2024-03-18

Imageset size:

550.18 GB

Imageset DOI:

10.6019/EMPIAR-11596

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe

Image Sets

1. Unaligned multi-frame micrographs of seeded Aβ(1-40) amyloid fibrils

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

3018

Image size:

(3838, 3710)

Pixel type:

UNSIGNED 16 BIT INTEGER

Pixel spacing:

(1.04, 1.04)

Details:

Krios1 Quantum-K2
Cs 2.7
300kV EFTEM nanpprobe
x130k spot 8 beam size 650nm
c2=50 objective_aperture=100

GIF-K2 counting
10s exposure 40 frames
total dose at the sample 53.79e/A2
1 pixel = 1.04A
dose rate ~4-5 e/pix/s

defocus range -0.8 to -2.0

The gain reference (/data/data/CountRef_map_0117_001.dm4) is not applied to the micrographs. The NOTES.txt file contains EM information. Thumbnails and power spectra (/data/thumbs) as well as coordinates (/data/manual pick) are provided.

Files:

This entry cannot be downloaded from PDBj.

Archives and downloads the selected files into an uncompressed zip file.

Depending on the number and size of files to be downloaded, this can take an enormous amount of time. Also, this feature is unstable and may not work properly, and checksums of downloaded files are not verified.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

Browse All Files

Quick Links

Talks and Tutorials

EMPIAR Quick tour

Statistics

Publications

Re-use case study

EMPIAR in the news

Claim entries to your ORCiD

COVID-19 Data Portal

EMDB

PDBe

BioImage Archive

EMNavigator@PDBj

PDBj

RCSB@PDBj

EMDataResoruce@RCSB,EMDB,NCMI

Citations

Small LEA proteins mitigate air-water interface damage to fragile cryo-EM samples during plunge freezing.

Abe KM, Li G, He Q, Grant T, Lim CJ. (2024)

Outer membrane protein assembly mediated by BAM-SurA complexes.

Fenn KL, Horne JE, Crossley JA, Böhringer N, Horne RJ, Schäberle TF, Calabrese AN, Radford SE, Ranson NA. (2024)

Ubiquitinated histone H2B as gatekeeper of the nucleosome acidic patch.

Hicks CW, Rahman S, Gloor SL, Fields JK, Husby NL, Vaidya A, Maier KE, Morgan M, Keogh MC, Wolberger C. (2024)

Molecular recognition of an odorant by the murine trace amine-associated receptor TAAR7f.

Gusach A, Lee Y, Khoshgrudi AN, Mukhaleva E, Ma N, Koers EJ, Chen Q, Edwards PC, Huang F, Kim J, Mancia F, Veprintsev DB, Vaidehi N, Weyand SN, Tate CG. (2024)

The novel ribosome biogenesis inhibitor usnic acid blocks nucleolar pre-60S maturation.

Kofler L, Grundmann L, Gerhalter M, Prattes M, Merl-Pham J, Zisser G, Grishkovskaya I, Hodirnau VV, Vareka M, Breinbauer R, Hauck SM, Haselbach D, Bergler H. (2024)

See all citations

Protein Data Bank Japan News

[2024-11-20]

224 new PDB entries have been released on 2024-11-20.

[2024-11-01]

A new portal integrating genome variants and protein structures has released

[2024-10-30]

[wwPDB] Deprecation of FTP File Download Protocol on November 1, 2024

[2024-10-25]

PDBj luncheon seminar at APBJC 2024

[2024-10-17]

[wwPDB] The Nobel Prize in Chemistry 2024