EMPIAR-11595
Unseeded Aβ(1-40) amyloid fibrils [4628 multi-frame micrographs composed of 918 frames each in EER format]
Publication:

Cryo-EM analysis of the effect of seeding with brain-derived Aβ amyloid fibrils

Pfeiffer PB, Ugrina M, Schwierz N, Sigurdson CJ, Schmidt M, Fändrich M

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Related EMDB entry:
Deposited:
2023-06-01
Released:
2024-03-18
Last modified:
2024-03-18
Imageset size:
1.88 TB
Imageset DOI:
Experimental metadata:
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Contains:
  • micrographs - multiframe
1. Unaligned multi-frame micrographs of unseeded Aβ(1-40) amyloid fibrils
Category:
micrographs - multiframe
Image format:
EER
No. of images or tilt series:
4628
Image size:
(4096, 4096)
Pixel type:
UNSIGNED 16 BIT INTEGER
Pixel spacing:
(0.934, 0.934)
Details:
IC-Krios1 G4 300kV C-FEG
SelectrisX 10eV slit width, Falcon4i
Cs=2.7mm, C2aperture=50um, ObjAperture=100um
EFTEM 130kx, nanoprobe spot 5, beam=630nm

Counting mode, 1pix=0.934 A
~2.9 s exposure, EER files save (918 fractions, not gain corrected)
~40 e/A^2 total dose

defocus range: -0.6 to -2.1

The gain reference (/data/movies/20220808_133943_EER_GainReference.gain) is not applied to the micrographs. The NOTES.txt file contains EM information. Thumbnails (/data/thumbs) as well as coordinates are provided. The coordinates are divided in morphology unseeded i (/data/manual pick unseeded i) and unseeded ii (/data/manual pick unseeded ii).
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