EMPIAR-11595 - Unseeded Aβ(1-40) amyloid fibrils [4628 multi-frame micrographs composed of 918 frames each in EER format]

EMPIAR-11595

Unseeded Aβ(1-40) amyloid fibrils [4628 multi-frame micrographs composed of 918 frames each in EER format]

Publication:

Cryo-EM analysis of the effect of seeding with brain-derived Aβ amyloid fibrils

Pfeiffer PB, Ugrina M, Schwierz N, Sigurdson CJ, Schmidt M, Fändrich M

(---)

Related EMDB entry:

EMD-17167 EMD-17166

Deposited:

2023-06-01

Released:

2024-03-18

Last modified:

2024-03-18

Imageset size:

1.88 TB

Imageset DOI:

10.6019/EMPIAR-11595

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe

Image Sets

1. Unaligned multi-frame micrographs of unseeded Aβ(1-40) amyloid fibrils

Category:

micrographs - multiframe

Image format:

EER

No. of images or tilt series:

4628

Image size:

(4096, 4096)

Pixel type:

UNSIGNED 16 BIT INTEGER

Pixel spacing:

(0.934, 0.934)

Details:

IC-Krios1 G4 300kV C-FEG
SelectrisX 10eV slit width, Falcon4i
Cs=2.7mm, C2aperture=50um, ObjAperture=100um
EFTEM 130kx, nanoprobe spot 5, beam=630nm

Counting mode, 1pix=0.934 A
~2.9 s exposure, EER files save (918 fractions, not gain corrected)
~40 e/A^2 total dose

defocus range: -0.6 to -2.1

The gain reference (/data/movies/20220808_133943_EER_GainReference.gain) is not applied to the micrographs. The NOTES.txt file contains EM information. Thumbnails (/data/thumbs) as well as coordinates are provided. The coordinates are divided in morphology unseeded i (/data/manual pick unseeded i) and unseeded ii (/data/manual pick unseeded ii).

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