EMPIAR-11574 - HIV-1 capsid lattice assembled on 30-nm liposome scaffolds in the presence of assembly cofactor IP6; bound to lenacapavir [696 multi-frame micrographs composed of 50 frames each in TIFF format]

EMPIAR-11574

HIV-1 capsid lattice assembled on 30-nm liposome scaffolds in the presence of assembly cofactor IP6; bound to lenacapavir [696 multi-frame micrographs composed of 50 frames each in TIFF format]

Publication:

Structural insights into HIV-1 polyanion-dependent capsid lattice formation revealed by single particle cryo-EM

Highland CM, Tan A, Ricaña CL, Briggs JAG, Dick RA

Proceedings of the National Academy of Sciences of the United States of America 120 (2023)

PMID: 37094124

DOI: 10.1073/pnas.2220545120

Related EMDB entry:

EMD-29776

Deposited:

2023-04-25

Released:

2023-06-19

Last modified:

2023-06-19

Imageset size:

1.82 TB

Imageset DOI:

10.6019/EMPIAR-11574

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe

Image Sets

1. Unaligned multi-frame micrographs of HIV-1 CA lattice templated on 30-nm liposomes in the presence of assembly cofactor IP6; bound to lenacapavir

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

696

Image size:

(11520, 8184)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(0.655, 0.655)

Details:

'Raw,' unaligned multi-frame movies collected using a Talos Arctica microscope equipped with a Gatan K3 detector/BioQuantum energy filter operating in super-resolution mode.

Voltage: 200 kV
Physical pixel size: 1.31Å/pixel
Total dose: 50 e-/Å^2

Gain reference (gain_ref.mrc) is included.
Picked particle coordinates are provided in run_data.star.

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This entry cannot be downloaded from PDBj.

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We recommend using rsync, aspera, globus, etc.

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Citations

Structural basis for HIV-1 antagonism of host APOBEC3G via Cullin E3 ligase.

Ito F, Alvarez-Cabrera AL, Liu S, Yang H, Shiriaeva A, Zhou ZH, Chen XS. (2023)

The 2023 Nucleic Acids Research Database Issue and the online molecular biology database collection.

Rigden DJ, Fernández XM. (2023)

EMPIAR: the Electron Microscopy Public Image Archive.

Iudin A, Korir PK, Somasundharam S, Weyand S, Cattavitello C, Fonseca N, Salih O, Kleywegt GJ, Patwardhan A. (2023)

CLEMSite, a software for automated phenotypic screens using light microscopy and FIB-SEM.

Serra Lleti JM, Steyer AM, Schieber NL, Neumann B, Tischer C, Hilsenstein V, Holtstrom M, Unrau D, Kirmse R, Lucocq JM, Pepperkok R, Schwab Y. (2023)

Structure of a RecT/Redβ family recombinase in complex with a duplex intermediate of DNA annealing.

Caldwell BJ, Norris AS, Karbowski CF, Wiegand AM, Wysocki VH, Bell CE. (2022)

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