EMPIAR-11549 - Cryo electron microscopy of assemblies based on truncated PVY coat protein with K176C mutation [9480 multi-frame micrographs composed of 32 frames each in TIFF format]

EMPIAR-11549

Cryo electron microscopy of assemblies based on truncated PVY coat protein with K176C mutation [9480 multi-frame micrographs composed of 32 frames each in TIFF format]

Publication:

From structural polymorphism to structural metamorphosis of the coat protein of flexuous filamentous potato virus Y

Kavčič L, Kežar A, Koritnik N, Žnidarič MT, Klobučar T, Vičič Ž, Merzel F, Holden E, Benesch JLP, Podobnik M

Communications chemistry 7 (2024)

PMID: 38233506

DOI: 10.1038/s42004-024-01100-x

Related EMDB entry:

EMD-17062 EMD-17063

Deposited:

2023-04-25

Released:

2024-02-29

Last modified:

2024-02-29

Imageset size:

957.10 GB

Imageset DOI:

10.6019/EMPIAR-11549

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe

Image Sets

1. Unaligned multi-frame micrographs of assemblies based on truncated PVY coat protein with K176C mutation

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

9480

Image size:

(3838, 3710)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(0.822, 0.822)

Details:

The movies were acquired (Titan Krios) on K2 Summit direct electron detector (Gatan) operating in counting mode at a nominal magnification of 165,000 corresponding to a pixel size of 0.822 Å. Each movie is collected across 32 frames with a total electron dose of 32 e-/Å2. Reference gain file (.dm4) and K2 camera defects file (.txt) are in the same directory.

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Gusach A, Lee Y, Khoshgrudi AN, Mukhaleva E, Ma N, Koers EJ, Chen Q, Edwards PC, Huang F, Kim J, Mancia F, Veprintsev DB, Vaidehi N, Weyand SN, Tate CG. (2024)

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