EMPIAR-11527
Single particle cryo-EM dataset of Mus musculus mitochondrial complex I [2674 multi-frame micrographs composed of 40 frames each in TIFF format]
Publication:

Investigation of hydrated channels and proton pathways in a high-resolution cryo-EM structure of mammalian complex I

Grba DN, Chung I, Bridges HR, Agip AA, Hirst J

Science advances 9 (2023)

PMID: 37531432

Related EMDB entry:
Deposited:
2023-04-24
Released:
2023-08-18
Last modified:
2023-08-18
Imageset size:
1.06 TB
Imageset DOI:
Experimental metadata:
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Contains:
  • micrographs - multiframe
1. Unaligned multi-frame micrographs of wild-type mouse mitochondrial complex I from an as-prepared sample solubilised in DDM
Category:
micrographs - multiframe
Image format:
TIFF
No. of images or tilt series:
2674
Image size:
(11520, 8184)
Pixel type:
UNSIGNED BYTE
Pixel spacing:
(0.5125, 0.5125)
Details:
The sample was frozen on PEGylated gold grids. Images were saved in super-resolution (11,520 x 8,184) at a value of 0.5125 Å but we later determined it to be 0.534 Å via calibration to previously obtained data and built models. The gain reference file (gainreference.mrc) and detector defects file (Defects.txt) are also uploaded.
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