EMPIAR-11523
CryoEM of E. coli RNA polymerase Sigma N bound to mismatch DNA fragment dhsU36mm1 (-12T) [multiple data sets in TIFF, MRC and MRCS formats]
Publication:

A general mechanism for transcription bubble nucleation in bacteria

Mueller AU, Chen J, Wu M, Chiu C, Nixon BT, Campbell EA, Darst SA

Proceedings of the National Academy of Sciences of the United States of America 120 (2023)

PMID: 36972428

Related EMDB entry:
Deposited:
2023-04-07
Released:
2023-05-16
Last modified:
2023-05-16
Imageset size:
4.44 TB
Imageset DOI:
Experimental metadata:
Download xml json
Contains:
  • micrographs - multiframe
  • micrographs - single frame
  • picked particles - single frame - processed
1. Unaligned micrographs (raw movies)
Category:
micrographs - multiframe
Image format:
TIFF
No. of images or tilt series:
12296
Image size:
(5760, 4092)
Pixel type:
UNSIGNED BYTE
Pixel spacing:
(1.083, 1.083)
Details:
FEI Titan Krios + Gatan K3, super-resolution, image shift, BioQuantum energy filter 20 eV slit; accelerating voltage 300 kV; dose 30 e-/px; pixel size 1.083 A/px; exposure 2.0 s; dose/frame 1.0231 e-/A^2/frame; frame time 0.05 s; total dose 51.16 e-/A^2; total frames 40; defocus range -1.0 to -2.5um
Gain references can be found in the "references" subfolder.
Picked particles star file "EsN-dhsU36mm1_particles_picked.star" ("aux_files" folder) contains all particles picked initially and is only provided for reference.
Files:
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2. Motion-corrected micrographs (dose-weighted)
Category:
micrographs - single frame
Image format:
MRC
No. of images or tilt series:
12296
Image size:
(5760, 4092)
Pixel type:
32 BIT FLOAT
Pixel spacing:
(1.083, 1.083)
Details:
See also the "corrected_micrographs.star" file in the "aux_files" folder.
Picked particles star file "EsN-dhsU36mm1_particles_picked.star" ("aux_files" folder) contains all particles picked initially and is only provided for reference.
Files:
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3. Particle images for map 1
Category:
picked particles - single frame - processed
Image format:
MRCS
No. of images or tilt series:
12140
Image size:
(256, 256)
Pixel type:
32 BIT FLOAT
Pixel spacing:
(1.083, 1.083)
Details:
Particle stack for map 1 after Bayesian polishing (see "EsN-dhsU36mm1_particles_map1.star" (final refinement parameters) and "EsN-dhsU36mm1_particles_shiny.star" (output from Bayesian Polishing) in "aux_files" folder)
Files:
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4. Particle images for map 2 (subtracted particles)
Category:
picked particles - single frame - processed
Image format:
MRCS
No. of images or tilt series:
1206
Image size:
(256, 256)
Pixel type:
32 BIT FLOAT
Pixel spacing:
(1.083, 1.083)
Details:
Particle stack for map 2 after masked subtraction (see "EsN-dhsU36mm1_particles_map2.star" (final refinement parameters) in "aux_files" folder; for original particles see "EsN-dhsU36mm1_particles_map1.star")
Files:
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Files:
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Citations

Ito F, Alvarez-Cabrera AL, Liu S, Yang H, Shiriaeva A, Zhou ZH, Chen XS. (2023)
Rigden DJ, Fernández XM. (2023)
Iudin A, Korir PK, Somasundharam S, Weyand S, Cattavitello C, Fonseca N, Salih O, Kleywegt GJ, Patwardhan A. (2023)
Serra Lleti JM, Steyer AM, Schieber NL, Neumann B, Tischer C, Hilsenstein V, Holtstrom M, Unrau D, Kirmse R, Lucocq JM, Pepperkok R, Schwab Y. (2023)
Caldwell BJ, Norris AS, Karbowski CF, Wiegand AM, Wysocki VH, Bell CE. (2022)