EMPIAR-11523 - CryoEM of E. coli RNA polymerase Sigma N bound to mismatch DNA fragment dhsU36mm1 (-12T) [multiple data sets in TIFF, MRC and MRCS formats]

EMPIAR-11523

CryoEM of E. coli RNA polymerase Sigma N bound to mismatch DNA fragment dhsU36mm1 (-12T) [multiple data sets in TIFF, MRC and MRCS formats]

Publication:

A general mechanism for transcription bubble nucleation in bacteria

Mueller AU, Chen J, Wu M, Chiu C, Nixon BT, Campbell EA, Darst SA

Proceedings of the National Academy of Sciences of the United States of America 120 (2023)

PMID: 36972428

DOI: 10.1073/pnas.2220874120

Related EMDB entry:

EMD-28797 EMD-28792 EMD-28794

Deposited:

2023-04-07

Released:

2023-05-16

Last modified:

2023-05-16

Imageset size:

4.44 TB

Imageset DOI:

10.6019/EMPIAR-11523

Experimental metadata:

Download xml

Contains:

micrographs - multiframe
micrographs - single frame
picked particles - single frame - processed

Image Sets

1. Unaligned micrographs (raw movies)

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

12296

Image size:

(5760, 4092)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(1.083, 1.083)

Details:

FEI Titan Krios + Gatan K3, super-resolution, image shift, BioQuantum energy filter 20 eV slit; accelerating voltage 300 kV; dose 30 e-/px; pixel size 1.083 A/px; exposure 2.0 s; dose/frame 1.0231 e-/A^2/frame; frame time 0.05 s; total dose 51.16 e-/A^2; total frames 40; defocus range -1.0 to -2.5um
Gain references can be found in the "references" subfolder.
Picked particles star file "EsN-dhsU36mm1_particles_picked.star" ("aux_files" folder) contains all particles picked initially and is only provided for reference.

Files:

2. Motion-corrected micrographs (dose-weighted)

Category:

micrographs - single frame

Image format:

MRC

No. of images or tilt series:

12296

Image size:

(5760, 4092)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(1.083, 1.083)

Details:

See also the "corrected_micrographs.star" file in the "aux_files" folder.
Picked particles star file "EsN-dhsU36mm1_particles_picked.star" ("aux_files" folder) contains all particles picked initially and is only provided for reference.

Files:

3. Particle images for map 1

Category:

picked particles - single frame - processed

Image format:

MRCS

No. of images or tilt series:

12140

Image size:

(256, 256)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(1.083, 1.083)

Details:

Particle stack for map 1 after Bayesian polishing (see "EsN-dhsU36mm1_particles_map1.star" (final refinement parameters) and "EsN-dhsU36mm1_particles_shiny.star" (output from Bayesian Polishing) in "aux_files" folder)

Files:

4. Particle images for map 2 (subtracted particles)

Category:

picked particles - single frame - processed

Image format:

MRCS

No. of images or tilt series:

1206

Image size:

(256, 256)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(1.083, 1.083)

Details:

Particle stack for map 2 after masked subtraction (see "EsN-dhsU36mm1_particles_map2.star" (final refinement parameters) in "aux_files" folder; for original particles see "EsN-dhsU36mm1_particles_map1.star")

Files: