EMPIAR-11513
Cryo-EM SPA datasets of broadly neutralizing antibody 2-36 in complex with prefusion SARS-CoV-2 and SARS-CoV spike glycoproteins (K3 movies/.tif files) [multiple data sets in TIFF format]
Publication:

A monoclonal antibody that neutralizes SARS-CoV-2 variants, SARS-CoV, and other sarbecoviruses

Wang P, Casner RG, Nair MS, Yu J, Guo Y, Wang M, Chan JF, Cerutti G, Iketani S, Liu L, Sheng Z, Chen Z, Yuen KY, Kwong PD, Huang Y, Shapiro L, Ho DD

Emerging microbes & infections 11 (2022) 147-157

PMID: 34836485

Related EMDB entry:
Deposited:
2022-11-16
Released:
2023-05-09
Last modified:
2023-05-09
Imageset size:
2.52 TB
Imageset DOI:
Experimental metadata:
Contains:
  • micrographs - multiframe
1. K3 Movies (.tif) for 2-36 Antibody + SARS-CoV-2 Spike
Category:
micrographs - multiframe
Image format:
TIFF
No. of images or tilt series:
4135
Image size:
(5760, 4092)
Pixel type:
UNSIGNED BYTE
Pixel spacing:
(1.07, 1.07)
Details:
Cryo-EM data for single particle analysis was collected on a Titan Krios electron microscope operating at 300 kV, equipped with an energy filter and a Gatan K3-BioQuantum direct detection detector, using the Leginon software package. Exposures were taken with a total electron fluence of 41.92 e-/Å2 fractionated over 60 frames, with a total exposure time of 3 s. A defocus range of −0.8 to −2.5 µm was used with a magnification of 81,000x, and a pixel size of 1.07 Å.
Files:
Loading...
2. K3 Movies (.tif) for 2-36 Antibody + SARS-CoV Spike
Category:
micrographs - multiframe
Image format:
TIFF
No. of images or tilt series:
4980
Image size:
(5760, 4092)
Pixel type:
UNSIGNED BYTE
Pixel spacing:
(1.07, 1.07)
Details:
Cryo-EM data for single particle analysis was collected on a Titan Krios electron microscope operating at 300 kV, equipped with an energy filter and a Gatan K3-BioQuantum direct detection detector, using the Leginon software package. Exposures were taken with a total electron fluence of 41.92 e-/Å2 fractionated over 60 frames, with a total exposure time of 3 s. A defocus range of −0.8 to −2.5 µm was used with a magnification of 81,000x, and a pixel size of 1.07 Å.
Files:
Loading...
Files:
Loading...
Quick Links
Citations
Ito F, Alvarez-Cabrera AL, Liu S, Yang H, Shiriaeva A, Zhou ZH, Chen XS. (2023)
Iudin A, Korir PK, Somasundharam S, Weyand S, Cattavitello C, Fonseca N, Salih O, Kleywegt GJ, Patwardhan A. (2023)
Serra Lleti JM, Steyer AM, Schieber NL, Neumann B, Tischer C, Hilsenstein V, Holtstrom M, Unrau D, Kirmse R, Lucocq JM, Pepperkok R, Schwab Y. (2023)
Caldwell BJ, Norris AS, Karbowski CF, Wiegand AM, Wysocki VH, Bell CE. (2022)
See all citations
Tweets::PDBj
Tweets::EMPIAR-EBI