EMPIAR-11513 - Cryo-EM SPA datasets of broadly neutralizing antibody 2-36 in complex with prefusion SARS-CoV-2 and SARS-CoV spike glycoproteins (K3 movies/.tif files) [multiple data sets in TIFF format]

EMPIAR-11513

Cryo-EM SPA datasets of broadly neutralizing antibody 2-36 in complex with prefusion SARS-CoV-2 and SARS-CoV spike glycoproteins (K3 movies/.tif files) [multiple data sets in TIFF format]

Publication:

A monoclonal antibody that neutralizes SARS-CoV-2 variants, SARS-CoV, and other sarbecoviruses

Wang P, Casner RG, Nair MS, Yu J, Guo Y, Wang M, Chan JF, Cerutti G, Iketani S, Liu L, Sheng Z, Chen Z, Yuen KY, Kwong PD, Huang Y, Shapiro L, Ho DD

Emerging microbes & infections 11 (2022) 147-157

PMID: 34836485

DOI: 10.1080/22221751.2021.2011623

Related EMDB entry:

EMD-24190

Deposited:

2022-11-16

Released:

2023-05-09

Last modified:

2023-05-09

Imageset size:

2.52 TB

Imageset DOI:

10.6019/EMPIAR-11513

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe

Image Sets

1. K3 Movies (.tif) for 2-36 Antibody + SARS-CoV-2 Spike

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

4135

Image size:

(5760, 4092)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(1.07, 1.07)

Details:

Cryo-EM data for single particle analysis was collected on a Titan Krios electron microscope operating at 300 kV, equipped with an energy filter and a Gatan K3-BioQuantum direct detection detector, using the Leginon software package. Exposures were taken with a total electron fluence of 41.92 e-/Å2 fractionated over 60 frames, with a total exposure time of 3 s. A defocus range of −0.8 to −2.5 µm was used with a magnification of 81,000x, and a pixel size of 1.07 Å.

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2. K3 Movies (.tif) for 2-36 Antibody + SARS-CoV Spike

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

4980

Image size:

(5760, 4092)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(1.07, 1.07)

Details:

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

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Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

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