EMPIAR-11502
Cryo-EM SPA dataset of 2-oxoglutarate dehydrogenase complex from a Chaetomium thermophilum native cell extract [25991 multi-frame micrographs composed of 13 frames each in MRC format]
Publication:

Structural analysis of an endogenous 4-megadalton succinyl-CoA-generating metabolon

Skalidis I, Kyrilis FL, Tüting C, Hamdi F, Träger TK, Belapure J, Hause G, Fratini M, O'Reilly FJ, Heilmann I, Rappsilber J, Kastritis PL

Communications biology 6 (2023)

PMID: 37217784

Related EMDB entry:
Deposited:
2023-02-13
Released:
2023-06-09
Last modified:
2023-06-09
Imageset size:
6.80 TB
Imageset DOI:
Experimental metadata:
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Compressed by PDBj:

Image formats and pixel types shown below are those of the original, non-compressed files.

MRC movies under the Movies directories in this entry were compressed by bzip2 (pbzip2)
by EMPIAR Japan to save space and bandwidth.

Some programs (e.g. RELION >= 4.0.1 and CryoSPARC) can process compressed movies without explicit
decompression. For other programs, movies have to be decompressed beforehand. In this case,
you might want to convert decompressed MRC movies into TIFF to save your local storage.

We realized that
EM_Data_PDC_Big_Dataset/20200317_F14_Fresh_Sample_obj_G3/20200317_F14_Fresh_Sample_obj_G3/
is a duplicate of the parent folder EM_Data_PDC_Big_Dataset/20200317_F14_Fresh_Sample_obj_G3.
We removed the redundant files.

Original size:   10,996,802,705 kilobytes
Compressed size:  5,790,206,905 kilobytes
Contains:
  • micrographs - multiframe
1. Cryo-EM SPA dataset of 2-oxoglutarate dehydrogenase complex from a Chaetomium thermophilum native cell extract
Category:
micrographs - multiframe
Image format:
MRC
No. of images or tilt series:
25991
Image size:
(4096, 4096)
Pixel type:
SIGNED 16 BIT INTEGER
Pixel spacing:
(1.5678, 1.5678)
Details:
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Serra Lleti JM, Steyer AM, Schieber NL, Neumann B, Tischer C, Hilsenstein V, Holtstrom M, Unrau D, Kirmse R, Lucocq JM, Pepperkok R, Schwab Y. (2023)
Caldwell BJ, Norris AS, Karbowski CF, Wiegand AM, Wysocki VH, Bell CE. (2022)