EMPIAR-11487
Single particle cryo-EM dataset of extended cowpea chlorotic mottle virus in plunge-frozen vitrified ice [multiple data sets in EER format]
Publication:

Fast viral dynamics revealed by microsecond time-resolved cryo-EM

Harder OF, Barrass SV, Drabbels M, Lorenz UJ

Nature communications 14 (2023)

PMID: 37704664

Related EMDB entry:
Deposited:
2023-03-01
Released:
2023-11-07
Last modified:
2023-11-07
Imageset size:
5.11 TB
Imageset DOI:
Experimental metadata:
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Contains:
  • micrographs - multiframe
1. Single particle cryo-EM dataset of extended cowpea chlorotic mottle virus in plunge-frozen vitrified ice, grid 1
Category:
micrographs - multiframe
Image format:
EER
No. of images or tilt series:
5391
Image size:
(4096, 4096)
Pixel type:
BIT
Pixel spacing:
(0.726, 0.726)
Details:
These are the EER files from Falcon 4 detector - raw micrographs, not motion corrected.

Samples of expanded CCMV are prepared at pH 7.6 in the absence of divalent ions and plunge frozen in the presence of a photoacid (NPE-caged proton). The pH of the sample is then lowered to 4.5 by releasing the photoacid with UV irradiation (266 nm).
Files:
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2. Single particle cryo-EM dataset of extended cowpea chlorotic mottle virus in plunge-frozen vitrified ice, grid 2
Category:
micrographs - multiframe
Image format:
EER
No. of images or tilt series:
5235
Image size:
(4096, 4096)
Pixel type:
BIT
Pixel spacing:
(0.726, 0.726)
Details:
These are the EER files from Falcon 4 detector - raw micrographs, not motion corrected.

Samples of expanded CCMV are prepared at pH 7.6 in the absence of divalent ions and plunge frozen in the presence of a photoacid (NPE-caged proton). The pH of the sample is then lowered to 4.5 by releasing the photoacid with UV irradiation (266 nm).
Files:
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