Unaligned, non-gain-normalised, 31-frame movies from a Bioquantum-K3 camera recorded in super-resolution mode with twofold binning at 130,000× magnification. 16553 movies were collected with EPU on the MIT.nano Titan Krios G3i operated at 300 kV. Each micrograph accumulates 40 electrons per square Angstrom over 31 frames (0.69 s). Nominal pixel size is 0.6632 Å/pixel. Gain reference image for the dataset is attached. Final refinement star files produced from focused refinements for each reconstruction deposited on EMDB are also attached: EMD-28064 at 3.0 Å resolution, and EMD-28070 at 3.2 Å resolution. Data was processed in RELION 4.0.
Further details about the dataset:
Grid: Quantifoil R1.2/1.3 on 300 copper mesh C2 aperture: 50 um Spot: 4 Beam diameter: 0.75 um EF slit: 20 eV Acquisition pattern: 4 shots/hole x 12 um AFIS Physical Pixel: 0.6632 A/pix SuperRes pixel: 0.3316 A/pix Exposure rate: 25.53 e/pix/s Exposure time: 0.69 s Total exposure: 40.07 e/A2 Frames: 31 Defocus: 0.5 - 2 um
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Unaligned, non-gain-normalised, 31-frame movies from a Bioquantum-K3 camera recorded in super-resolution mode with twofold binning at 130,000× magnification. 32890 movies were collected with EPU on the MIT.nano Titan Krios G3i operated at 300 kV. Each micrograph accumulates 40 electrons per square Angstrom over 31 frames (0.69 s). Nominal pixel size is 0.6632 Å/pixel. Gain reference image for the dataset is attached. Final refinement star files produced from focused refinements for each reconstruction deposited on EMDB are also attached: EMD-28065 at 2.5 Å resolution, EMD-28071 at 2.8 Å resolution, EMD-28072 at 2.6 Å resolution, and EMD-28073 at 2.7 Å resolution. Only one-third of the dataset was used during processing in RELION 4.0.
Further details about the dataset:
Grid: Quantifoil R1.2/1.3 on 300 copper mesh C2 aperture: 50 um Spot: 4 Beam diameter: 0.75 um EF slit: 20 eV Acquisition pattern: 4 shots/hole x 12 um AFIS Physical Pixel: 0.6632 A/pix SuperRes pixel: 0.3316 A/pix Exposure rate: 25.53 e/pix/s Exposure time: 0.69 s Total exposure: 40.07 e/A2 Frames: 31 Defocus: 0.5 - 2 um
Files:
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This entry cannot be downloaded from PDBj.
Archives and downloads the selected files into an uncompressed zip file.
Depending on the number and size of files to be downloaded, this can take an enormous amount of time.
Also, this feature is unstable and may not work properly, and checksums of downloaded files are not verified.
We recommend using rsync, aspera, globus, etc.
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Archives and downloads the selected files into an uncompressed zip file.
Depending on the number and size of files to be downloaded, this can take an enormous amount of time.
Also, this feature is unstable and may not work properly, and checksums of downloaded files are not verified.
We recommend using rsync, aspera, globus, etc.
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It is possible to download files by specifying the file list with rsync command, etc.
