Tilt-series were acquired using ThermoFisher Titan Krios microscopes operated at 300 keV, equipped with a K3 camera and Quantum energy filter in zeros-loss mode. The energy slit width was set to 20 eV. The tilt-series were collected with serialEM using a dose-symmetric scheme, starting from 0° with a 3° tilt increment by a group of 3, with an angular range from -60° to 60°. The accumulated exposure for each tilt-series was ~120 e-/Å2, with a defocus range between -2 and -5 µm. The micrographs have been motion-corrected and stacked using IMOD newstack command. The dataset contains the motion-corrected tilt-series, transformation matrix file (*.xf), local transformation file (*local), tilt angle (*tlt) and gold beads positions (*erase), which are generated from IMOD.
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