EMPIAR-11442 - SJ25C1 Fab in complex with soluble CD19 [multiple data sets in TIFF format]

EMPIAR-11442

SJ25C1 Fab in complex with soluble CD19 [multiple data sets in TIFF format]

Publication:

CD19 CAR antigen engagement mechanisms and affinity tuning

He C, Mansilla-Soto J, Khanra N, Hamieh M, Bustos V, Paquette AJ, Garcia Angus A, Shore DM, Rice WJ, Khelashvili G, Sadelain M, Meyerson JR

Science immunology 8 (2023)

PMID: 36867678

DOI: 10.1126/sciimmunol.adf1426

Related EMDB entry:

EMD-26720

Deposited:

2023-02-22

Released:

2024-03-18

Last modified:

2024-03-18

Imageset size:

3.81 TB

Imageset DOI:

10.6019/EMPIAR-11442

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe

Image Sets

1. SJ25C1 Fab in complex with soluble CD19 dataset 1

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

3733

Image size:

(11520, 8184)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(0.4296, 0.4296)

Details:

Arctica operated at 200 kV with a GatanK3 imaging system collected at 45,000X nominal magnification. The calibrated pixel size of 0.4296 Å was used for processing.
Movies were collected using Leginon (Suloway et al., 2005) at a dose rate of 33.42 e-/Å2/s with a total exposure of 1.60 seconds, for an accumulated dose of 53.47 e-/Å2. Intermediate frames were recorded every 0.04 seconds for a total of 40 frames per micrograph.

Files:

This entry cannot be downloaded from PDBj.

Archives and downloads the selected files into an uncompressed zip file.

Depending on the number and size of files to be downloaded, this can take an enormous amount of time. Also, this feature is unstable and may not work properly, and checksums of downloaded files are not verified.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

2. SJ25C1 Fab in complex with soluble CD19 dataset 2

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

5021

Image size:

(11520, 8184)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(0.4296, 0.4296)

Details:

Arctica operated at 200 kV with a GatanK3 imaging system collected at 45,000X nominal magnification. The calibrated pixel size of 0.4296 Å was used for processing.
Movies were collected using Leginon (Suloway et al., 2005) at a dose rate of 33.78 e-/Å2/s with a total exposure of 1.60 seconds, for an accumulated dose of 54.04 e-/Å2. Intermediate frames were recorded every 0.04 seconds for a total of 40 frames per micrograph.

Files:

This entry cannot be downloaded from PDBj.

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

Browse All Files

Quick Links

Talks and Tutorials

EMPIAR Quick tour

Statistics

Publications

Re-use case study

EMPIAR in the news

Claim entries to your ORCiD

COVID-19 Data Portal

EMDB

PDBe

BioImage Archive

EMNavigator@PDBj

PDBj

RCSB@PDBj

EMDataResoruce@RCSB,EMDB,NCMI

Citations

Small LEA proteins mitigate air-water interface damage to fragile cryo-EM samples during plunge freezing.

Abe KM, Li G, He Q, Grant T, Lim CJ. (2024)

Outer membrane protein assembly mediated by BAM-SurA complexes.

Fenn KL, Horne JE, Crossley JA, Böhringer N, Horne RJ, Schäberle TF, Calabrese AN, Radford SE, Ranson NA. (2024)

Ubiquitinated histone H2B as gatekeeper of the nucleosome acidic patch.

Hicks CW, Rahman S, Gloor SL, Fields JK, Husby NL, Vaidya A, Maier KE, Morgan M, Keogh MC, Wolberger C. (2024)

Molecular recognition of an odorant by the murine trace amine-associated receptor TAAR7f.

Gusach A, Lee Y, Khoshgrudi AN, Mukhaleva E, Ma N, Koers EJ, Chen Q, Edwards PC, Huang F, Kim J, Mancia F, Veprintsev DB, Vaidehi N, Weyand SN, Tate CG. (2024)

The novel ribosome biogenesis inhibitor usnic acid blocks nucleolar pre-60S maturation.

Kofler L, Grundmann L, Gerhalter M, Prattes M, Merl-Pham J, Zisser G, Grishkovskaya I, Hodirnau VV, Vareka M, Breinbauer R, Hauck SM, Haselbach D, Bergler H. (2024)

See all citations

Protein Data Bank Japan News

[2024-11-20]

224 new PDB entries have been released on 2024-11-20.

[2024-11-01]

A new portal integrating genome variants and protein structures has released

[2024-10-30]

[wwPDB] Deprecation of FTP File Download Protocol on November 1, 2024

[2024-10-25]

PDBj luncheon seminar at APBJC 2024

[2024-10-17]

[wwPDB] The Nobel Prize in Chemistry 2024