EMPIAR-11412 - Molecular mechanism of Trypanosoma brucei Aquaporin 2 with pentamidine [multiple data sets in MRC and MRCS formats]

EMPIAR-11412

Molecular mechanism of Trypanosoma brucei Aquaporin 2 with pentamidine [multiple data sets in MRC and MRCS formats]

Publication:

Insights from aquaporin structures into drug-resistant sleeping sickness

Matusevicius M, Corey RA, Gragera M, Yamashita K, Sprenger T, Ungogo MA, Blaza JM, Castro-Hartmann P, Chirgadze DY, Vedithi SC, Afanasyev P, Melero R, Warshamanage R, Gusach A, Carazo JM, Carrington M, Blundell TM, Murshudov G, Stansfeld P, Sansom M, De Koning HP, Tate CG, Weyand SN

eLife RP107460 (2025)

DOI: 10.7554/eLife.107460.1

Related EMDB entry:

EMD-16863

Deposited:

2023-02-04

Released:

2025-10-27

Last modified:

2025-10-27

Imageset size:

957.44 GB

Imageset DOI:

10.6019/EMPIAR-11412

Experimental metadata:

Download xml json

Compressed by PDBj:

Image formats and pixel types shown below are those of the original, non-compressed files.

--- file not found ---

Contains:

micrographs - single frame
picked particles - single frame - processed
micrographs - multiframe

Image Sets

1. Aligned dose-weighted micrographs of Aqp2 + pentamidine from Trypanosoma brucei (MotionCorr2 output)

Category:

micrographs - single frame

Image format:

MRC

No. of images or tilt series:

3263

Image size:

(3838, 3710)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(1.07, 1.07)

Details:

Aligned (all frames) and dose-weighted micrographs from MotionCorr2

Files:

Archives and downloads the selected files into an uncompressed zip file.

Depending on the number and size of files to be downloaded, this can take an enormous amount of time. Also, this feature is unstable and may not work properly, and checksums of downloaded files are not verified.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

2. Particle set of the final 3D reconstruction of Aqp2 + pentamidine from Trypanosoma brucei

Category:

picked particles - single frame - processed

Image format:

MRCS

No. of images or tilt series:

83845

Image size:

(320, 320)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(1.07, 1.07)

Details:

Particles that yielded the final 3D reconstruction of Aqp2 + pentamidine.

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

3. Unaligned multiframe movies of Aqp2 + pentamidine from Trypanosoma brucei

Category:

micrographs - multiframe

Image format:

MRC

No. of images or tilt series:

3262

Image size:

(3838, 3710)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(1.07, 1.07)

Details:

---

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

Browse All Files

Quick Links

Browse EMPIAR

Sample Preparation Widget

Volume Browser

Tomo Viewer Prototype

Claim entries to your ORCiD

Talks and Tutorials

EMPIAR Quick tour

Publications

Re-use study

Statistics

EMPIAR in the news

COVID-19 Data Portal

EMDB

BioImage Archive

PDBe

EMNavigator@PDBj

PDBj

Citations

CryoSIP: unleashing protein high-resolution Cryo-EM via semantic-instance collaborative picking.

Deng Y, Wang S, Xiang M, Li Y, Zhuo L, Cao D, Fu X, Zou Q. (2026)

prismPYP: Power-spectrum and image domain learning for self-supervised micrograph evaluation.

He L, Bartesaghi A. (2026)

Unbend, correction of local beam-induced sample motion in cryo-EM images using a 3D spline model.

Kong L, Zottig X, Elferich J, Grigorieff N. (2026)

Dual site targeting of the bacterial 70S ribosome by tetracyclines.

Devarkar SC, Lomakin IB, Wang J, Grada A, Bunick CG. (2026)

Chart builder: an interactive tool for user driven data visualization in the electron microscopy data bank.

Fonseca N, Duraisamy AK, Wang Z, Somasundharam S, Tayebinia M, de Oliveira LC, Ma M, Turner J, Patwardhan A, Kleywegt GJ, Hartley M, Morris KL. (2026)

Deep-learning methods for contrast enhancement and artifact reduction in cryo-electron tomography: a systematic analysis of the state of the art and proposed improvements.

Jones HN, Deshmukh A, Pande K. (2026)

CryoVirusDB: An Annotated Dataset for AI-Based Virus Particle Identification in Cryo-EM Micrographs.

Gyawali R, Dhakal A, Wang L, Cheng J. (2026)

CryoGS: High-Quality Cryo-EM Homogeneous Reconstruction by Gaussian Splatting

Zeng B, Liu S, Cheng S, Xu G, Fan H. (2026)

Point cloud deformation modeling for particle selection following cryo-EM 2D classification.

Wang X, Mo Z, Li F, Zhang F, Wan X. (2026)

CryoSift: an accessible and automated CNN-driven tool for cryo-EM 2D class selection.

Schäfer JH, Calza A, Hom K, Damodar P, Peng R, Bogdanović N, Lander GC, Stagg SM, Cianfrocco MA. (2025)

See all citations

Protein Data Bank Japan News