EMPIAR-11412
Molecular mechanism of Trypanosoma brucei Aquaporin 2 with pentamidine [multiple data sets in MRC and MRCS formats]
Publication:

Insights from aquaporin structures into drug-resistant sleeping sickness

Matusevicius M, Corey RA, Gragera M, Yamashita K, Sprenger T, Ungogo MA, Blaza JM, Castro-Hartmann P, Chirgadze DY, Vedithi SC, Afanasyev P, Melero R, Warshamanage R, Gusach A, Carazo JM, Carrington M, Blundell TM, Murshudov G, Stansfeld P, Sansom M, De Koning HP, Tate CG, Weyand SN

eLife RP107460 (2025)

Related EMDB entry:
Deposited:
2023-02-04
Released:
2025-10-27
Last modified:
2025-10-27
Imageset size:
957.44 GB
Imageset DOI:
Experimental metadata:
Download xml json

Compressed by PDBj:

Image formats and pixel types shown below are those of the original, non-compressed files.

Movies (Export/FoilHole*.mrc) in this entry were compressed to TIFF by EMPIAR Japan to save space and bandwidth.
The compression is lossless in that all pixel intensities remain exactly the same after decompression.
However, some metadata in the MRC header were lost.
The original files before compression are available from EMPIAR EBI.

Compression was carried out by separating (“un-normalizing”) the gain.
The details of the method are described in
https://relion.readthedocs.io/en/release-3.1/Reference/MovieCompression.html#gain-normalised-movies.

Please apply the gain reference (Export/gain-reference.mrc) during processing.

PDBj discovered that this entry contained an ascp binary (`Export/ascp`) and removed it.
PDBj also discovered that three movies in `Export/pentamidine` were duplicates of the
same files in `Export` and removed them.

Original size:   7,257,434,604 kilobytes
Compressed size:   788,940,215 kilobytes
Contains:
  • micrographs - single frame
  • picked particles - single frame - processed
  • micrographs - multiframe
1. Aligned dose-weighted micrographs of Aqp2 + pentamidine from Trypanosoma brucei (MotionCorr2 output)
Category:
micrographs - single frame
Image format:
MRC
No. of images or tilt series:
3263
Image size:
(3838, 3710)
Pixel type:
32 BIT FLOAT
Pixel spacing:
(1.07, 1.07)
Details:
Aligned (all frames) and dose-weighted micrographs from MotionCorr2
Files:
Loading...
2. Particle set of the final 3D reconstruction of Aqp2 + pentamidine from Trypanosoma brucei
Category:
picked particles - single frame - processed
Image format:
MRCS
No. of images or tilt series:
83845
Image size:
(320, 320)
Pixel type:
32 BIT FLOAT
Pixel spacing:
(1.07, 1.07)
Details:
Particles that yielded the final 3D reconstruction of Aqp2 + pentamidine.
Files:
Loading...
3. Unaligned multiframe movies of Aqp2 + pentamidine from Trypanosoma brucei
Category:
micrographs - multiframe
Image format:
MRC
No. of images or tilt series:
3262
Image size:
(3838, 3710)
Pixel type:
32 BIT FLOAT
Pixel spacing:
(1.07, 1.07)
Details:
---
Files:
Loading...
He L, Bartesaghi A. (2026)
Deng Y, Wang S, Xiang M, Li Y, Zhuo L, Cao D, Fu X, Zou Q. (2026)
Fonseca N, Duraisamy AK, Wang Z, Somasundharam S, Tayebinia M, de Oliveira LC, Ma M, Turner J, Patwardhan A, Kleywegt GJ, Hartley M, Morris KL. (2026)
Gyawali R, Dhakal A, Wang L, Cheng J. (2026)
Jones HN, Deshmukh A, Pande K. (2026)
Zeng B, Liu S, Cheng S, Xu G, Fan H. (2026)
Wang X, Mo Z, Li F, Zhang F, Wan X. (2026)
Kishore V, Debarnot V, Righetto RD, Engel BD, Dokmanić I. (2026)
Schäfer JH, Calza A, Hom K, Damodar P, Peng R, Bogdanović N, Lander GC, Stagg SM, Cianfrocco MA. (2025)
Geißler K, Kreysing JP, Wang Y, Glushkova D, Obarska-Kosinska A, Hoffmann PC, Böhm S, Schmidt A, Meier-Credo J, Langer JD, Hummer G, Beck M. (2026)