EMPIAR-11410 - Molecular mechanism of Trypanosoma brucei Aquaporin 2 with Glycerol [multiple data sets in MRC and MRCS formats]

EMPIAR-11410

Molecular mechanism of Trypanosoma brucei Aquaporin 2 with Glycerol [multiple data sets in MRC and MRCS formats]

Publication:

Insights from aquaporin structures into drug-resistant sleeping sickness

Matusevicius M, Corey RA, Gragera M, Yamashita K, Sprenger T, Ungogo MA, Blaza JM, Castro-Hartmann P, Chirgadze DY, Vedithi SC, Afanasyev P, Melero R, Warshamanage R, Gusach A, Carazo JM, Carrington M, Blundell TM, Murshudov G, Stansfeld P, Sansom M, De Koning HP, Tate CG, Weyand SN

eLife RP107460 (2025)

DOI: 10.7554/eLife.107460.1

Related EMDB entry:

EMD-16864

Deposited:

2022-09-24

Released:

2025-10-28

Last modified:

2025-10-28

Imageset size:

3.03 TB

Imageset DOI:

10.6019/EMPIAR-11410

Experimental metadata:

Download xml json

Compressed by PDBj:

Image formats and pixel types shown below are those of the original, non-compressed files.

--- file not found ---

Contains:

micrographs - single frame
picked particles - single frame - processed
micrographs - multiframe

Image Sets

1. Aligned micrographs of Aqp2 from Trypanosoma brucei with glycerol (MotionCorr2 output)

Category:

micrographs - single frame

Image format:

MRC

No. of images or tilt series:

2882

Image size:

(4096, 4096)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(1.07, 1.07)

Details:

Aligned (all frames) and dose-weighted micrographs from MotionCorr2.

Files:

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2. Particle set of the final 3D reconstruction of Aqp2 + glycerol from Trypanosoma brucei

Category:

picked particles - single frame - processed

Image format:

MRCS

No. of images or tilt series:

100075

Image size:

(320, 320)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(1.07, 1.07)

Details:

Particles from final 3D reconstruction.

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

3. Aligned micrographs of Aqp2 from Trypanosoma brucei with glycerol (MotionCorr2 output)

Category:

micrographs - multiframe

Image format:

MRCS

No. of images or tilt series:

2881

Image size:

(4096, 4096)

Pixel type:

SIGNED 16 BIT INTEGER

Pixel spacing:

(1.07, 1.07)

Details:

---

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

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Citations

prismPYP: Power-spectrum and image domain learning for self-supervised micrograph evaluation.

He L, Bartesaghi A. (2026)

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Deng Y, Wang S, Xiang M, Li Y, Zhuo L, Cao D, Fu X, Zou Q. (2026)

Unbend, correction of local beam-induced sample motion in cryo-EM images using a 3D spline model.

Kong L, Zottig X, Elferich J, Grigorieff N. (2026)

Chart builder: an interactive tool for user driven data visualization in the electron microscopy data bank.

Fonseca N, Duraisamy AK, Wang Z, Somasundharam S, Tayebinia M, de Oliveira LC, Ma M, Turner J, Patwardhan A, Kleywegt GJ, Hartley M, Morris KL. (2026)

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Devarkar SC, Lomakin IB, Wang J, Grada A, Bunick CG. (2026)

CryoVirusDB: An Annotated Dataset for AI-Based Virus Particle Identification in Cryo-EM Micrographs.

Gyawali R, Dhakal A, Wang L, Cheng J. (2026)

Deep-learning methods for contrast enhancement and artifact reduction in cryo-electron tomography: a systematic analysis of the state of the art and proposed improvements.

Jones HN, Deshmukh A, Pande K. (2026)

CryoGS: High-Quality Cryo-EM Homogeneous Reconstruction by Gaussian Splatting

Zeng B, Liu S, Cheng S, Xu G, Fan H. (2026)

Point cloud deformation modeling for particle selection following cryo-EM 2D classification.

Wang X, Mo Z, Li F, Zhang F, Wan X. (2026)

ICECREAM: high-fidelity equivariant cryo-electron tomography.

Kishore V, Debarnot V, Righetto RD, Engel BD, Dokmanić I. (2026)