EMPIAR-11397
CryoEM micrographs of mouse apoferritin in a range of ice thicknesses on different microscope setups [multiple data sets in MRC and TIFF formats]
Publication:

Measuring the effects of ice thickness on resolution in single particle cryo-EM

Neselu K, Wang B, Rice WJ, Potter CS, Carragher B, Chua EYD

Journal of structural biology: X 7 (2023)

PMID: 36742017

Deposited:
2022-11-30
Released:
2023-02-27
Last modified:
2023-02-27
Imageset size:
5.67 TB
Imageset DOI:
Experimental metadata:
Download xml json
Contains:
  • micrographs - single frame
  • micrographs - multiframe
1. Aligned and dose weighted micrographs of mouse apoferritin sorted by ice thickness collected on a 200 kV Glacios microscope with a Falcon3 detector in integrating mode
Category:
micrographs - single frame
Image format:
MRC
No. of images or tilt series:
3474
Image size:
(4096, 4096)
Pixel type:
32 BIT FLOAT
Pixel spacing:
(1.204, 1.204)
Details:
Micrographs were aligned and dose weighted in MotionCor2. Here, they have been sorted into their respective ice thickness bins, 0-50 nm, 50-100 nm, 100-150 nm, 150-200 nm, and 200-500 nm. The obj_icegraph.txt file lists all micrographs collected during the session and the corresponding ice thickness measured by aperture limited scattering.
Files:
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2. Aligned and dose weighted micrographs of mouse apoferritin sorted by energy filtered state and ice thickness collected on a 300 kV Krios microscope with a K3 detector in counting mode
Category:
micrographs - single frame
Image format:
MRC
No. of images or tilt series:
14803
Image size:
(5760, 4092)
Pixel type:
32 BIT FLOAT
Pixel spacing:
(1.083, 1.083)
Details:
Micrographs were aligned and dose weighted in MotionCor2. Here, they have been sorted into energy filtered state (with or without 20 eV energy filter) and respective ice thickness bins, 0-50 nm, 50-100 nm, 100-150 nm, 150-200 nm, and 200-500 nm. The zlp_icegraph.txt file lists all micrographs collected during the session and the corresponding ice thickness measured by energy filtration.
Files:
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3. Aligned and dose weighted micrographs of mouse apoferritin collected on a 200 kV Arctica microscope with a K3 detector sorted by counting or super resolution mode and ice thickness
Category:
micrographs - single frame
Image format:
MRC
No. of images or tilt series:
8666
Image size:
(5760, 4092)
Pixel type:
32 BIT FLOAT
Pixel spacing:
(1.096, 1.096)
Details:
Micrographs were aligned and dose weighted in MotionCor2. Here, they have been sorted into detector state (counting or super resolution) and respective ice thickness bins, 0-50 nm, 50-100 nm, 100-150 nm, 150-200 nm, and 200-500 nm. The obj_icegraph.txt or icethickness.txt file lists all micrographs collected during the session and the corresponding ice thickness measured by aperture limited scattering.
Files:
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4. Raw movie frames of mouse apoferritin collected on a 200 kV Arctica microscope with a K3 detector in counting mode
Category:
micrographs - multiframe
Image format:
TIFF
No. of images or tilt series:
5781
Image size:
(5760, 4092)
Pixel type:
UNSIGNED BYTE
Pixel spacing:
(1.096, 1.096)
Details:
These are the raw, unaligned movie frames. The obj_icegraph.txt file in the parent directory lists all micrographs collected during the session and the corresponding ice thickness measured by aperture limited scattering.
Files:
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5. Raw movie frames of mouse apoferritin collected on a 200 kV Arctica microscope with a K3 detector in super resolution mode
Category:
micrographs - multiframe
Image format:
TIFF
No. of images or tilt series:
2895
Image size:
(11520, 8184)
Pixel type:
UNSIGNED BYTE
Pixel spacing:
(0.548, 0.548)
Details:
These are the raw, unaligned movie frames. The icethickness.txt file in the parent directory lists all micrographs collected during the session and the corresponding ice thickness measured by aperture limited scattering.
Files:
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Files:
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