EMPIAR-11349
Cryo-EM structure of a delivery complex containing the SspB adaptor, an ssrA-tagged substrate, and the AAA+ ClpXP protease [9524 multi-frame micrographs composed of 40 frames each in TIFF format]
Publication:

The SspB adaptor drives structural changes in the AAA+ClpXP protease during ssrA-tagged substrate delivery

Ghanbarpour A, Fei X, Baker TA, Davis JH, Sauer RT

bioRxiv (2022)

Related EMDB entry:
Deposited:
2022-11-30
Released:
2023-01-31
Last modified:
2023-01-31
Imageset size:
4.02 TB
Imageset DOI:
Experimental metadata:
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Contains:
  • micrographs - multiframe
1. Unaligned Movies of a Delivery Complex Containing the SspB Adaptor, an ssrA-Tagged Substrate, and the AAA+ ClpXP Protease
Category:
micrographs - multiframe
Image format:
TIFF
No. of images or tilt series:
9524
Image size:
(11520, 8184)
Pixel type:
UNSIGNED BYTE
Pixel spacing:
(0.435, 0.435)
Details:
Nominal Defocus range (min, max, step): -1.0, -2.5, -0.25
Total exposure on specimen: 75.98 el/A2
Exposure time: 2.02 s
Nominal pixel size: 0.87 A (0.435 A super res)
Calibrated pixel size: 0.832 (0.416 A super res)
Objective lens spherical aberration (Cs): 2.7 mm
Acceleration voltage: 300 kV
Extraction voltage: 3550 V
Microscope / Detector: Krios G3i / K3
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