Structure of RecT protein from Listeria innoccua phage A118 in complex with 83-mer annealed duplex [2038 multi-frame micrographs composed of 36 frames each in MRC format]
Publication:
Structure of a RecT/Redβ family recombinase in complex with a duplex intermediate of DNA annealing
Data collected on 9/17/2020-9/18-2020 Krios, K3, counted mode, EFTEM, Cs corrector User/Group:Brian Caldwell/Chuck Bell
Image information: Total # of images = 2038 Total data size = 1.70 TB Rate of collection = 71/hour Mode of collection = Accurate hole centering Gain normalized = YES File format = mrc Super-resolution = NO Spherical aberration = 0.01 mm Images per hole = 1 Exposure time = 2.7 s Fractions = 36 [usually 1-2 electrons per fractions]
Microscope parameters: Pixel size = 0.899 A Magnification = 81,000 Stage tilt = 0 deg Dose rate = 19.644 e/px/s Dose rate = 24.28 e/A^2/s Total dose = 65 e/A^2 [desired dose rate] Defocus range = -3.5, -3, -2.5, -2, -1.5, -1 C2 aperture = 50 um Objective aperture= 100 um Illuminated area = 1.5 um Energy Filter = YES Slit width = 20 eV
Grid type = Quantifoil (gold coated) R1.2/1.3 300 Mesh hole size, mesh, etc. Position 1 of the large dewar Bell Conical: Position 1 of box J2 (Gray) Prepared on 8/20/2020 by Brian Caldwell
Additional notes: Copied gain reference files over into the data folder. Saved EPU settings file.
Files:
Available download options:
Archives and downloads the selected files into an uncompressed zip file.
Depending on the number and size of files to be downloaded, this can take an enormous amount of time.
Also, this feature is unstable and may not work properly, and checksums of downloaded files are not verified.
We recommend using rsync, aspera, globus, etc.
Download a list of selected files.
It is possible to download files by specifying the file list with rsync command, etc.
Archives and downloads the selected files into an uncompressed zip file.
Depending on the number and size of files to be downloaded, this can take an enormous amount of time.
Also, this feature is unstable and may not work properly, and checksums of downloaded files are not verified.
We recommend using rsync, aspera, globus, etc.
Download a list of selected files.
It is possible to download files by specifying the file list with rsync command, etc.