EMPIAR-11338 - Cryo-EM SPA dataset for Antibody SKV09 in complex with VEEV VLP (K3 movies/.tif files) [5595 multi-frame micrographs composed of 40 frames each in TIFF format]

EMPIAR-11338

Cryo-EM SPA dataset for Antibody SKV09 in complex with VEEV VLP (K3 movies/.tif files) [5595 multi-frame micrographs composed of 40 frames each in TIFF format]

Publication:

Vaccine elicitation and structural basis for antibody protection against alphaviruses

Sutton MS, Pletnev S, Callahan V, Ko S, Tsybovsky Y, Bylund T, Casner RG, Cerutti G, Gardner CL, Guirguis V, Verardi R, Zhang B, Ambrozak D, Beddall M, Lei H, Yang ES, Liu T, Henry AR, Rawi R, Schön A, Schramm CA, Shen CH, Shi W, Stephens T, Yang Y, Florez MB, Ledgerwood JE, Burke CW, Shapiro L, Fox JM, Kwong PD, Roederer M

Cell 186 (2023) 2672-2689.e25

PMID: 37295404

DOI: 10.1016/j.cell.2023.05.019

Related EMDB entry:

EMD-27395

Deposited:

2022-12-02

Released:

2023-07-07

Last modified:

2023-07-07

Imageset size:

776.68 GB

Imageset DOI:

10.6019/EMPIAR-11338

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe

Image Sets

1. K3 Movies (.tif) for SKV09 Antibody + VEEV VLP

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

5595

Image size:

(5760, 4092)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(0.83, 0.83)

Details:

Cryo-EM data for single particle analysis were collected at the Columbia Cryo-EM Facility on a Titan Krios electron microscope operating at 300 kV, equipped with a Gatan K3-BioQuantum direct detection detector and energy filter, using the Leginon software package. Exposures were taken at a magnification of 105,000x (pixel size of 0.83 Å), using a total electron fluence of 46.5 e-/Å2 fractionated over 40 frames with an exposure time of 2.0 s. A random defocus range of -0.8 to -2.0 µm was used.

Files:

This entry cannot be downloaded from PDBj.

Archives and downloads the selected files into an uncompressed zip file.

Depending on the number and size of files to be downloaded, this can take an enormous amount of time. Also, this feature is unstable and may not work properly, and checksums of downloaded files are not verified.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

Browse All Files

Files:

This entry cannot be downloaded from PDBj.

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

Quick Links

Talks and Tutorials

EMPIAR Quick tour

Statistics

Publications

Re-use case study

EMPIAR in the news

Claim entries to your ORCiD

COVID-19 Data Portal

EMDB

PDBe

BioImage Archive

EMNavigator@PDBj

PDBj

RCSB@PDBj

EMDataResoruce@RCSB,EMDB,NCMI

Citations

Structural basis for HIV-1 antagonism of host APOBEC3G via Cullin E3 ligase.

Ito F, Alvarez-Cabrera AL, Liu S, Yang H, Shiriaeva A, Zhou ZH, Chen XS. (2023)

The 2023 Nucleic Acids Research Database Issue and the online molecular biology database collection.

Rigden DJ, Fernández XM. (2023)

EMPIAR: the Electron Microscopy Public Image Archive.

Iudin A, Korir PK, Somasundharam S, Weyand S, Cattavitello C, Fonseca N, Salih O, Kleywegt GJ, Patwardhan A. (2023)

CLEMSite, a software for automated phenotypic screens using light microscopy and FIB-SEM.

Serra Lleti JM, Steyer AM, Schieber NL, Neumann B, Tischer C, Hilsenstein V, Holtstrom M, Unrau D, Kirmse R, Lucocq JM, Pepperkok R, Schwab Y. (2023)

Structure of a RecT/Redβ family recombinase in complex with a duplex intermediate of DNA annealing.

Caldwell BJ, Norris AS, Karbowski CF, Wiegand AM, Wysocki VH, Bell CE. (2022)

See all citations

Protein Data Bank Japan News

[2024-05-01]

288 new PDB entries have been released on 2024-05-01.

[2024-04-09]

[wwPDB] CASP16 Call for Targets

[2024-04-02]

Announcement of the PDBj Newsletter Vol.24 release

[2024-03-21]

[wwPDB] Paper Published on NMR Restraint Validation

[2024-03-13]

[wwPDB] Paper Published on CryoEM Archiving and Validation Recommendations

[2024-03-06]

[wwPDB] Poster Prize Awarded at The Biophysical Society Meeting

[2024-02-15]

Notice of Service Suspension (ASH, DASH, SeSAW, MAFFTash, Spanner, SFAS)

[2024-02-13]

[wwPDB] Biocurator Milestone: >10,000 Depositions Processed

[2024-02-06]

[wwPDB] Preprint Published on NMR Restraint Validation

[2024-02-05]

[wwPDB] Preprint Published on CryoEM Archiving and Validation Recommendations

See all news