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Electron Microscopy Public Image Archive

EMPIAR-11314
CLEMSite, a software for automated phenotypic screens using light microscopy and FIB-SEM. [multiple data sets in TIFF format]
Publication:

CLEMSite, a software for automated phenotypic screens using light microscopy and FIB-SEM.

Lleti JMSL, Steyer AMS, Schieber NLS, Neumann BN, Tischer CT, Hilsenstein VH, Holtstrom MH, Unrau DU, Kirmse RK, Lucocq JML, Pepperkok RP, Schwab YS

Journal of Cell Biology (---)

Related EMDB entry:
Deposited:
2022-11-21
Released:
2022-12-19
Last modified:
2022-12-19
Imageset size:
19.74 GB
Imageset DOI:
10.6019/EMPIAR-11314
Experimental metadata:
Download xml json
Contains:
  • micrographs - multiframe
Image Sets
1. A1_COPB1
Category:
micrographs - multiframe
Image format:
TIFF
No. of images or tilt series:
147
Image size:
(4260, 1218)
Pixel type:
UNSIGNED BYTE
Pixel spacing:
(80.0, 80.0)
Details:
Automatically acquired FIB-SEM dataset of GalNAc-T2 cell with voxel size 8x8x200 nm. Knockdown of COPB1 with liquid phase transfection for 48h, afterwards chemically fixed and embedded in Durcupan. Image stack inverted and aligned with SIFT in Fiji.
Files:
Loading...
2. A2_COPB1
Category:
micrographs - multiframe
Image format:
TIFF
No. of images or tilt series:
147
Image size:
(4254, 1479)
Pixel type:
UNSIGNED BYTE
Pixel spacing:
(80.0, 80.0)
Details:
Automatically acquired FIB-SEM dataset of GalNAc-T2 cell with voxel size 8x8x200 nm. Knockdown of COPB1 with liquid phase transfection for 48h, afterwards chemically fixed and embedded in Durcupan. Image stack inverted and aligned with SIFT in Fiji.
Files:
Loading...
3. A3_COPB1
Category:
micrographs - multiframe
Image format:
TIFF
No. of images or tilt series:
147
Image size:
(3810, 1236)
Pixel type:
UNSIGNED BYTE
Pixel spacing:
(80.0, 80.0)
Details:
Automatically acquired FIB-SEM dataset of GalNAc-T2 cell with voxel size 8x8x200 nm. Knockdown of COPB1 with liquid phase transfection for 48h, afterwards chemically fixed and embedded in Durcupan. Image stack inverted and aligned with SIFT in Fiji.
Files:
Loading...
4. A4_COPB1
Category:
micrographs - multiframe
Image format:
TIFF
No. of images or tilt series:
147
Image size:
(4151, 1173)
Pixel type:
UNSIGNED BYTE
Pixel spacing:
(80.0, 80.0)
Details:
Automatically acquired FIB-SEM dataset of GalNAc-T2 cell with voxel size 8x8x200 nm. Knockdown of COPB1 with liquid phase transfection for 48h, afterwards chemically fixed and embedded in Durcupan. Image stack inverted and aligned with SIFT in Fiji.
Files:
Loading...
5. A5_COPB1
Category:
micrographs - multiframe
Image format:
TIFF
No. of images or tilt series:
61
Image size:
(3695, 1179)
Pixel type:
UNSIGNED BYTE
Pixel spacing:
(80.0, 80.0)
Details:
Automatically acquired FIB-SEM dataset of GalNAc-T2 cell with voxel size 8x8x200 nm. Knockdown of COPB1 with liquid phase transfection for 48h, afterwards chemically fixed and embedded in Durcupan. Image stack inverted and aligned with SIFT in Fiji.
Files:
Loading...
6. B1_COPB1
Category:
micrographs - multiframe
Image format:
TIFF
No. of images or tilt series:
152
Image size:
(2946, 1416)
Pixel type:
UNSIGNED BYTE
Pixel spacing:
(80.0, 80.0)
Details:
Automatically acquired FIB-SEM dataset of GalNAc-T2 cell with voxel size 8x8x200 nm. Knockdown of COPB1 with liquid phase transfection for 48h, afterwards chemically fixed and embedded in Durcupan. Image stack inverted and aligned with SIFT in Fiji.
Files:
Loading...
7. B2_COPB1
Category:
micrographs - multiframe
Image format:
TIFF
No. of images or tilt series:
152
Image size:
(3531, 1266)
Pixel type:
UNSIGNED BYTE
Pixel spacing:
(80.0, 80.0)
Details:
Automatically acquired FIB-SEM dataset of GalNAc-T2 cell with voxel size 8x8x200 nm. Knockdown of COPB1 with liquid phase transfection for 48h, afterwards chemically fixed and embedded in Durcupan. Image stack inverted and aligned with SIFT in Fiji.
Files:
Loading...
8. B3_COPB1
Category:
micrographs - multiframe
Image format:
TIFF
No. of images or tilt series:
148
Image size:
(4266, 2268)
Pixel type:
UNSIGNED BYTE
Pixel spacing:
(80.0, 80.0)
Details:
Automatically acquired FIB-SEM dataset of GalNAc-T2 cell with voxel size 8x8x200 nm. Knockdown of COPB1 with liquid phase transfection for 48h, afterwards chemically fixed and embedded in Durcupan. Image stack inverted and aligned with SIFT in Fiji.
Files:
Loading...
9. B4_COPB1
Category:
micrographs - multiframe
Image format:
TIFF
No. of images or tilt series:
147
Image size:
(3804, 1488)
Pixel type:
UNSIGNED BYTE
Pixel spacing:
(80.0, 80.0)
Details:
Automatically acquired FIB-SEM dataset of GalNAc-T2 cell with voxel size 8x8x200 nm. Knockdown of COPB1 with liquid phase transfection for 48h, afterwards chemically fixed and embedded in Durcupan. Image stack inverted and aligned with SIFT in Fiji.
Files:
Loading...
10. B5_COPB1
Category:
micrographs - multiframe
Image format:
TIFF
No. of images or tilt series:
147
Image size:
(4197, 1326)
Pixel type:
UNSIGNED BYTE
Pixel spacing:
(80.0, 80.0)
Details:
Automatically acquired FIB-SEM dataset of GalNAc-T2 cell with voxel size 8x8x200 nm. Knockdown of COPB1 with liquid phase transfection for 48h, afterwards chemically fixed and embedded in Durcupan. Image stack inverted and aligned with SIFT in Fiji.
Files:
Loading...
11. C1_COPB1
Category:
micrographs - multiframe
Image format:
TIFF
No. of images or tilt series:
147
Image size:
(4200, 1353)
Pixel type:
UNSIGNED BYTE
Pixel spacing:
(80.0, 80.0)
Details:
Automatically acquired FIB-SEM dataset of GalNAc-T2 cell with voxel size 8x8x200 nm. Knockdown of COPB1 with liquid phase transfection for 48h, afterwards chemically fixed and embedded in Durcupan. Image stack inverted and aligned with SIFT in Fiji.
Files:
Loading...
12. C2_COPB1
Category:
micrographs - multiframe
Image format:
TIFF
No. of images or tilt series:
122
Image size:
(4316, 1221)
Pixel type:
UNSIGNED BYTE
Pixel spacing:
(80.0, 80.0)
Details:
Automatically acquired FIB-SEM dataset of GalNAc-T2 cell with voxel size 8x8x200 nm. Knockdown of COPB1 with liquid phase transfection for 48h, afterwards chemically fixed and embedded in Durcupan. Image stack inverted and aligned with SIFT in Fiji.
Files:
Loading...
13. C3_COPB1
Category:
micrographs - multiframe
Image format:
TIFF
No. of images or tilt series:
147
Image size:
(3981, 1542)
Pixel type:
UNSIGNED BYTE
Pixel spacing:
(80.0, 80.0)
Details:
Automatically acquired FIB-SEM dataset of GalNAc-T2 cell with voxel size 8x8x200 nm. Knockdown of COPB1 with liquid phase transfection for 48h, afterwards chemically fixed and embedded in Durcupan. Image stack inverted and aligned with SIFT in Fiji.
Files:
Loading...
14. C4_COPB1
Category:
micrographs - multiframe
Image format:
TIFF
No. of images or tilt series:
147
Image size:
(4319, 1218)
Pixel type:
UNSIGNED BYTE
Pixel spacing:
(80.0, 80.0)
Details:
Automatically acquired FIB-SEM dataset of GalNAc-T2 cell with voxel size 8x8x200 nm. Knockdown of COPB1 with liquid phase transfection for 48h, afterwards chemically fixed and embedded in Durcupan. Image stack inverted and aligned with SIFT in Fiji.
Files:
Loading...
15. C5_COPB1
Category:
micrographs - multiframe
Image format:
TIFF
No. of images or tilt series:
147
Image size:
(4313, 1413)
Pixel type:
UNSIGNED BYTE
Pixel spacing:
(80.0, 80.0)
Details:
Automatically acquired FIB-SEM dataset of GalNAc-T2 cell with voxel size 8x8x200 nm. Knockdown of COPB1 with liquid phase transfection for 48h, afterwards chemically fixed and embedded in Durcupan. Image stack inverted and aligned with SIFT in Fiji.
Files:
Loading...
16. D1_COPB1
Category:
micrographs - multiframe
Image format:
TIFF
No. of images or tilt series:
147
Image size:
(4099, 1353)
Pixel type:
UNSIGNED BYTE
Pixel spacing:
(80.0, 80.0)
Details:
Automatically acquired FIB-SEM dataset of GalNAc-T2 cell with voxel size 8x8x200 nm. Knockdown of COPB1 with liquid phase transfection for 48h, afterwards chemically fixed and embedded in Durcupan. Image stack inverted and aligned with SIFT in Fiji.
Files:
Loading...
17. D2_COPB1
Category:
micrographs - multiframe
Image format:
TIFF
No. of images or tilt series:
147
Image size:
(4200, 1785)
Pixel type:
UNSIGNED BYTE
Pixel spacing:
(80.0, 80.0)
Details:
Automatically acquired FIB-SEM dataset of GalNAc-T2 cell with voxel size 8x8x200 nm. Knockdown of COPB1 with liquid phase transfection for 48h, afterwards chemically fixed and embedded in Durcupan. Image stack inverted and aligned with SIFT in Fiji.
Files:
Loading...
18. D3_COPB1
Category:
micrographs - multiframe
Image format:
TIFF
No. of images or tilt series:
147
Image size:
(4319, 1863)
Pixel type:
UNSIGNED BYTE
Pixel spacing:
(80.0, 80.0)
Details:
Automatically acquired FIB-SEM dataset of GalNAc-T2 cell with voxel size 8x8x200 nm. Knockdown of COPB1 with liquid phase transfection for 48h, afterwards chemically fixed and embedded in Durcupan. Image stack inverted and aligned with SIFT in Fiji.
Files:
Loading...
19. D4_COPB1
Category:
micrographs - multiframe
Image format:
TIFF
No. of images or tilt series:
146
Image size:
(4376, 1773)
Pixel type:
UNSIGNED BYTE
Pixel spacing:
(80.0, 80.0)
Details:
Automatically acquired FIB-SEM dataset of GalNAc-T2 cell with voxel size 8x8x200 nm. Knockdown of COPB1 with liquid phase transfection for 48h, afterwards chemically fixed and embedded in Durcupan. Image stack inverted and aligned with SIFT in Fiji.
Files:
Loading...
20. D5_COPB1
Category:
micrographs - multiframe
Image format:
TIFF
No. of images or tilt series:
150
Image size:
(3872, 1479)
Pixel type:
UNSIGNED BYTE
Pixel spacing:
(80.0, 80.0)
Details:
Automatically acquired FIB-SEM dataset of GalNAc-T2 cell with voxel size 8x8x200 nm. Knockdown of COPB1 with liquid phase transfection for 48h, afterwards chemically fixed and embedded in Durcupan. Image stack inverted and aligned with SIFT in Fiji.
Files:
Loading...
21. E1_COPB1
Category:
micrographs - multiframe
Image format:
TIFF
No. of images or tilt series:
147
Image size:
(4203, 1173)
Pixel type:
UNSIGNED BYTE
Pixel spacing:
(80.0, 80.0)
Details:
Automatically acquired FIB-SEM dataset of GalNAc-T2 cell with voxel size 8x8x200 nm. Knockdown of COPB1 with liquid phase transfection for 48h, afterwards chemically fixed and embedded in Durcupan. Image stack inverted and aligned with SIFT in Fiji.
Files:
Loading...
22. E2_COPB1
Category:
micrographs - multiframe
Image format:
TIFF
No. of images or tilt series:
153
Image size:
(3324, 2136)
Pixel type:
UNSIGNED BYTE
Pixel spacing:
(80.0, 80.0)
Details:
Automatically acquired FIB-SEM dataset of GalNAc-T2 cell with voxel size 8x8x200 nm. Knockdown of COPB1 with liquid phase transfection for 48h, afterwards chemically fixed and embedded in Durcupan. Image stack inverted and aligned with SIFT in Fiji.
Files:
Loading...
23. E3_COPB1
Category:
micrographs - multiframe
Image format:
TIFF
No. of images or tilt series:
146
Image size:
(4203, 1341)
Pixel type:
UNSIGNED BYTE
Pixel spacing:
(80.0, 80.0)
Details:
Automatically acquired FIB-SEM dataset of GalNAc-T2 cell with voxel size 8x8x200 nm. Knockdown of COPB1 with liquid phase transfection for 48h, afterwards chemically fixed and embedded in Durcupan. Image stack inverted and aligned with SIFT in Fiji.
Files:
Loading...
24. E4_COPB1
Category:
micrographs - multiframe
Image format:
TIFF
No. of images or tilt series:
147
Image size:
(4099, 1281)
Pixel type:
UNSIGNED BYTE
Pixel spacing:
(80.0, 80.0)
Details:
Automatically acquired FIB-SEM dataset of GalNAc-T2 cell with voxel size 8x8x200 nm. Knockdown of COPB1 with liquid phase transfection for 48h, afterwards chemically fixed and embedded in Durcupan. Image stack inverted and aligned with SIFT in Fiji.
Files:
Loading...
25. E5_COPB1
Category:
micrographs - multiframe
Image format:
TIFF
No. of images or tilt series:
147
Image size:
(4251, 1428)
Pixel type:
UNSIGNED BYTE
Pixel spacing:
(80.0, 80.0)
Details:
Automatically acquired FIB-SEM dataset of GalNAc-T2 cell with voxel size 8x8x200 nm. Knockdown of COPB1 with liquid phase transfection for 48h, afterwards chemically fixed and embedded in Durcupan. Image stack inverted and aligned with SIFT in Fiji.
Files:
Loading...
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