EMPIAR-11311
Cryo-EM structure of the mutant solitary ECF module 2EQ in MSP2N2 lipid nanodiscs in the ATPase closed and ATP-bound conformation (300 kV) [multiple data sets in TIFF format]
Publication:

Expulsion mechanism of the substrate-translocating subunit in ECF transporters

Thangaratnarajah C, Nijland M, Borges-Araújo L, Jeucken A, Rheinberger J, Marrink SJ, Souza PCT, Paulino C, Slotboom DJ

Nature communications 14 (2023)

PMID: 37491368

Related EMDB entry:
Deposited:
2022-11-15
Released:
2023-09-06
Last modified:
2023-09-06
Imageset size:
4.41 TB
Imageset DOI:
Experimental metadata:
Download xml json
Contains:
  • micrographs - multiframe
1. Unaligned movie frames (Dataset 1)
Category:
micrographs - multiframe
Image format:
TIFF
No. of images or tilt series:
9932
Image size:
(5760, 4092)
Pixel type:
UNSIGNED BYTE
Pixel spacing:
(0.836, 0.836)
Details:
Unaligned multi-frame movies with each containing 75 frames per movie collected from a Quantifoil Au 1.2/1.3 300 mesh grid at 300 kV in super-resolution counting mode with hardware binning by a factor of 2 (Counted Super Resolution Bin 2). Total accumulated electron exposure on the specimen level corresponds to 60 electrons per Å^2. Movies are not gain corrected and the gain reference is provided. Data acquired with EPU and Aberration Free Image Shift (AFIS) enabled.
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2. Unaligned movie frames (Dataset 2)
Category:
micrographs - multiframe
Image format:
TIFF
No. of images or tilt series:
5326
Image size:
(5760, 4092)
Pixel type:
UNSIGNED BYTE
Pixel spacing:
(0.836, 0.836)
Details:
Unaligned multi-frame movies with each containing 75 frames per movie collected from a Quantifoil Au 1.2/1.3 300 mesh grid at 300 kV in super-resolution counting mode with hardware binning by a factor of 2 (Counted Super Resolution Bin 2). Total accumulated electron exposure on the specimen level corresponds to 60 electrons per Å^2. Movies are not gain corrected and the gain reference is provided. Data acquired with EPU and Aberration Free Image Shift (AFIS) enabled.
Files:
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Files:
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