EMPIAR-11306 - Cryo-electron tomography on plasma FIB lamellae of HeLa cells [6481 tilt series in EER format]

EMPIAR-11306

Cryo-electron tomography on plasma FIB lamellae of HeLa cells [6481 tilt series in EER format]

Publication:

Plasma FIB milling for the determination of structures in situ

Berger C, Dumoux M, Glen T, Yee NB, Mitchels JM, Patáková Z, Darrow MC, Naismith JH, Grange M

Nature communications 14 (2023)

PMID: 36746945

DOI: 10.1038/s41467-023-36372-9

Related EMDB entry:

EMD-15636

Deposited:

2022-11-10

Released:

2023-02-03

Last modified:

2023-02-03

Imageset size:

622.19 GB

Imageset DOI:

10.6019/EMPIAR-11306

Experimental metadata:

Download xml json

Contains:

tilt series

Image Sets

1. Tilt-series recorded on argon PFIB lamellae of HeLa cells

Category:

tilt series

Image format:

EER

No. of images or tilt series:

6481

Image size:

(4096, 4096)

Pixel type:

4 BIT INTEGER

Pixel spacing:

(1.9, 1.9)

Details:

Tilt-series recorded on argon PFIB lamellae of HeLa cells using a dose-symmetric tilt-scheme corrected for the pre-tilt of the lamellae. Electron dose: 5 e/A2 per tilt. Data recorded at a pixel size of 1.85 A/px, which was refined to 1.9A/px based on the Subtomogram average structure.

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