EMPIAR-11286 - Cryo-EM Structure of Formate Dehydrogenase 1 from Methylorubrum extorquens AM1 [7650 multi-frame micrographs composed of 40 frames each in TIFF format]

EMPIAR-11286

Cryo-EM Structure of Formate Dehydrogenase 1 from Methylorubrum extorquens AM1 [7650 multi-frame micrographs composed of 40 frames each in TIFF format]

Publication:

Multiple electron transfer pathways of tungsten-containing formate dehydrogenase in direct electron transfer-type bioelectrocatalysis

Yoshikawa T, Makino F, Miyata T, Suzuki Y, Tanaka H, Namba K, Kano K, Sowa K, Kitazumi Y, Shirai O

Chemical communications (Cambridge, England) 58 (2022) 6478-6481

PMID: 35535582

DOI: 10.1039/d2cc01541b

Related EMDB entry:

EMD-32151

Deposited:

2022-10-25

Released:

2022-11-28

Last modified:

2022-11-28

Imageset size:

1.44 TB

Imageset DOI:

10.6019/EMPIAR-11286

Experimental metadata:

Download xml json

Deposited via PDBj

Contains:

micrographs - multiframe

Image Sets

1. Raw movies

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

7650

Image size:

(5760, 4092)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(0.87, 0.87)

Details:

300 kV
Cs 2.7 mm
2.0 e/Å2/frame

Note that the pixel size in the image header (0.767 Å/px) is wrong. 0.87 Å/px is the calibrated value.

Please use CountCDSRef_FoDH_5mg_Au_45-2_000.mrc as a gain reference without rotation or flipping.

The last three digits (000 to 074) in the movie file names represent the beam shift directions: 5 x 5 (holes) x 3 (images per hole). The preceding digits (e.g. 48-22) are the stage positions.

Files:

Archives and downloads the selected files into an uncompressed zip file.

Depending on the number and size of files to be downloaded, this can take an enormous amount of time. Also, this feature is unstable and may not work properly, and checksums of downloaded files are not verified.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

Browse All Files

Quick Links

Talks and Tutorials

EMPIAR Quick tour

Statistics

Publications

Re-use case study

EMPIAR in the news

Claim entries to your ORCiD

COVID-19 Data Portal

EMDB

PDBe

BioImage Archive

EMNavigator@PDBj

PDBj

RCSB@PDBj

EMDataResoruce@RCSB,EMDB,NCMI

Citations

Small LEA proteins mitigate air-water interface damage to fragile cryo-EM samples during plunge freezing.

Abe KM, Li G, He Q, Grant T, Lim CJ. (2024)

Outer membrane protein assembly mediated by BAM-SurA complexes.

Fenn KL, Horne JE, Crossley JA, Böhringer N, Horne RJ, Schäberle TF, Calabrese AN, Radford SE, Ranson NA. (2024)

Ubiquitinated histone H2B as gatekeeper of the nucleosome acidic patch.

Hicks CW, Rahman S, Gloor SL, Fields JK, Husby NL, Vaidya A, Maier KE, Morgan M, Keogh MC, Wolberger C. (2024)

Molecular recognition of an odorant by the murine trace amine-associated receptor TAAR7f.

Gusach A, Lee Y, Khoshgrudi AN, Mukhaleva E, Ma N, Koers EJ, Chen Q, Edwards PC, Huang F, Kim J, Mancia F, Veprintsev DB, Vaidehi N, Weyand SN, Tate CG. (2024)

The novel ribosome biogenesis inhibitor usnic acid blocks nucleolar pre-60S maturation.

Kofler L, Grundmann L, Gerhalter M, Prattes M, Merl-Pham J, Zisser G, Grishkovskaya I, Hodirnau VV, Vareka M, Breinbauer R, Hauck SM, Haselbach D, Bergler H. (2024)

See all citations

Protein Data Bank Japan News

[2024-11-20]

224 new PDB entries have been released on 2024-11-20.

[2024-11-01]

A new portal integrating genome variants and protein structures has released

[2024-10-30]

[wwPDB] Deprecation of FTP File Download Protocol on November 1, 2024

[2024-10-25]

PDBj luncheon seminar at APBJC 2024

[2024-10-17]

[wwPDB] The Nobel Prize in Chemistry 2024