EMPIAR-11252 - Cryo-EM snapshots of Eukaryotic Membrane Proteins in Native Lipid-Bilayer Nanodiscs [multiple data sets in MRC format]

EMPIAR-11252

Cryo-EM snapshots of Eukaryotic Membrane Proteins in Native Lipid-Bilayer Nanodiscs [multiple data sets in MRC format]

Publication:

Cryo-Electron Microscopy Snapshots of Eukaryotic Membrane Proteins in Native Lipid-Bilayer Nanodiscs

Janson K, Kyrilis FL, Tüting C, Alfes M, Das M, Träger TK, Schmidt C, Hamdi F, Vargas C, Keller S, Meister A, Kastritis PL

Biomacromolecules 23 (2022) 5084-5094

PMID: 36399657

DOI: 10.1021/acs.biomac.2c00935

Related EMDB entry:

EMD-15517 EMD-15516

Deposited:

2022-08-01

Released:

2023-02-13

Last modified:

2023-02-13

Imageset size:

5.71 TB

Imageset DOI:

10.6019/EMPIAR-11252

Experimental metadata:

Download xml json

Compressed by PDBj:

Image formats and pixel types shown below are those of the original, non-compressed files.

MRC movies (FoilHole*Fractions.mrc) in this entry were compressed by bzip2 (pbzip2)
by EMPIAR Japan to save space and bandwidth.

Some programs (e.g. RELION >= 4.0.1 and CryoSPARC) can process compressed movies without explicit
decompression. For other programs, movies have to be decompressed beforehand. In this case,
you might want to convert decompressed MRC movies into TIFF to save your local storage.

Original size:   13,994,564,558 kilobytes
Compressed size:  6,126,677,048 kilobytes

Contains:

micrographs - multiframe

Image Sets

1. Thin-ice acquisition of native nanodiscs from Chaetomium thermophilum

Category:

micrographs - multiframe

Image format:

MRC

No. of images or tilt series:

1207

Image size:

(4096, 4096)

Pixel type:

SIGNED 16 BIT INTEGER

Pixel spacing:

(0.5918, 0.5918)

Details:

High resolution multi-frame micrographs of native nanodiscs in thin ice. Due to the ice thickness, top and bottom views are clearly visible.

Files:

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2. Thick-ice acquisition of native nanodiscs from Chaetomium thermophilum

Category:

micrographs - multiframe

Image format:

MRC

No. of images or tilt series:

5913

Image size:

(4096, 4096)

Pixel type:

SIGNED BYTE

Pixel spacing:

(0.5918, 0.5918)

Details:

High resolution multi-frame micrographs of native nanodiscs in thicker ice. Due to the thicker ice, nanodiscs and proteins are present in all rotational orientations without preference.

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

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Browse All Files

Files:

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We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

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