EMPIAR-11215
Focused ion beam-scanning electron microscopy links pathological myelin outfoldings to axonal changes in mice lacking Plp1 or Mag [1151 micrographs in TIFF format]
Publication:

Focused ion beam-scanning electron microscopy links pathological myelin outfoldings to axonal changes in mice lacking Plp1 or Mag

Steyer AM, Buscham TJ, Lorenz C, Hümmert S, Eichel-Vogel MA, Schadt LC, Edgar JM, Köster S, Möbius W, Nave KA, Werner HB

Glia (2022)

PMID: 36354016

Related EMDB entry:
Deposited:
2022-09-15
Released:
2022-12-13
Last modified:
2022-12-13
Imageset size:
17.83 GB
Imageset DOI:
Experimental metadata:
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Contains:
  • micrographs - single frame
1. FIB-SEM of mouse optic nerve of Plp knock-out (Plp-/Y-4017)
Category:
micrographs - single frame
Image format:
TIFF
No. of images or tilt series:
1151
Image size:
(3984, 4176)
Pixel type:
UNSIGNED BYTE
Pixel spacing:
(50.0, 50.0)
Details:
Optic nerve of mice harboring null-alleles of the Plp/Plp1 (Plp-/Y) sarificed by cervical dislocation at postnatal day 75 (P75). The optic nerves were dissected and immersion fixed and prepared using the reduced osmium tetroxide – thiocarbohydrazide - osmium (OTO) method as previously described in detail (Weil et al. 2018; Steyer et al. 2020) upon implementing modifications to the original protocol first introduced by (Deerinck et al. 2010). The micrographs are obtained by FIB-SEM (Zeiss Crossbeam 540): SEM: 1.5 kV (600 pA), ESB grid 450V, pixel size x/y 5 nm, continuous mill-and-acquire mode; FIB: 1.5 nA , z-step 25 nm. Postprocessing: alignment with Fiji/ImageJ SIFT, cropped, inverted and blurred (Gaussian blur, sigma 2), local contrast enhancement (127, 55, 1.5) and sharpened with Fiji/ImageJ.
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