EMPIAR-11211 - Single-particle cryo-EM dataset of ALC1 bound to an asymmetric, site-specifically PARylated nucleosome [multiple data sets in TIFF and MRCS formats]

EMPIAR-11211

Single-particle cryo-EM dataset of ALC1 bound to an asymmetric, site-specifically PARylated nucleosome [multiple data sets in TIFF and MRCS formats]

Publication:

TBD

Bacic L

(---)

PMID: 38307862

DOI: 10.1038/s41467-024-45237-8

Related EMDB entry:

EMD-15777

Deposited:

2022-09-06

Released:

2023-09-21

Last modified:

2023-09-21

Imageset size:

8.31 TB

Imageset DOI:

10.6019/EMPIAR-11211

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe
picked particles - single frame - processed

Image Sets

1. Raw movies from grid 1

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

13668

Image size:

(5760, 4092)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(0.8617, 0.8617)

Details:

Movies from grid 1 and final set of particles from both grids.

Files:

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2. Raw movies from grid 2

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

8487

Image size:

(5760, 4092)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(0.8617, 0.8617)

Details:

Movies from grid 2 and final set of particles from both grids.

Files:

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3. Final set of particles after refinement in cryoSPARC

Category:

picked particles - single frame - processed

Image format:

MRCS

No. of images or tilt series:

212256

Image size:

(256, 256)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(0.8617, 0.8617)

Details:

Final set of particles that produced the map in EMD-15777 after refinement in cryoSPARC.

Files:

This entry cannot be downloaded from PDBj.

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4. Intermediate set of particles after classification in RELION, before 3DVA clustering in cryoSPARC

Category:

picked particles - single frame - processed

Image format:

MRCS

No. of images or tilt series:

739042

Image size:

(256, 256)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(0.8617, 0.8617)

Details:

Intermediate set of particles obtained after classification in RELION. This was the input of cryoSPARC 3DVA and clustering.

Files:

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Browse All Files

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