EMPIAR-11211
Single-particle cryo-EM dataset of ALC1 bound to an asymmetric, site-specifically PARylated nucleosome [multiple data sets in TIFF and MRCS formats]
Publication:
Related EMDB entry:
Deposited:
2022-09-06
Released:
2023-09-21
Last modified:
2023-09-21
Imageset size:
8.31 TB
Imageset DOI:
Experimental metadata:
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Contains:
  • micrographs - multiframe
  • picked particles - single frame - processed
1. Raw movies from grid 1
Category:
micrographs - multiframe
Image format:
TIFF
No. of images or tilt series:
13668
Image size:
(5760, 4092)
Pixel type:
UNSIGNED BYTE
Pixel spacing:
(0.8617, 0.8617)
Details:
Movies from grid 1 and final set of particles from both grids.
Files:
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2. Raw movies from grid 2
Category:
micrographs - multiframe
Image format:
TIFF
No. of images or tilt series:
8487
Image size:
(5760, 4092)
Pixel type:
UNSIGNED BYTE
Pixel spacing:
(0.8617, 0.8617)
Details:
Movies from grid 2 and final set of particles from both grids.
Files:
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3. Final set of particles after refinement in cryoSPARC
Category:
picked particles - single frame - processed
Image format:
MRCS
No. of images or tilt series:
212256
Image size:
(256, 256)
Pixel type:
32 BIT FLOAT
Pixel spacing:
(0.8617, 0.8617)
Details:
Final set of particles that produced the map in EMD-15777 after refinement in cryoSPARC.
Files:
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4. Intermediate set of particles after classification in RELION, before 3DVA clustering in cryoSPARC
Category:
picked particles - single frame - processed
Image format:
MRCS
No. of images or tilt series:
739042
Image size:
(256, 256)
Pixel type:
32 BIT FLOAT
Pixel spacing:
(0.8617, 0.8617)
Details:
Intermediate set of particles obtained after classification in RELION. This was the input of cryoSPARC 3DVA and clustering.
Files:
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Abe KM, Li G, He Q, Grant T, Lim CJ. (2024)
Fenn KL, Horne JE, Crossley JA, Böhringer N, Horne RJ, Schäberle TF, Calabrese AN, Radford SE, Ranson NA. (2024)
Hicks CW, Rahman S, Gloor SL, Fields JK, Husby NL, Vaidya A, Maier KE, Morgan M, Keogh MC, Wolberger C. (2024)
Gusach A, Lee Y, Khoshgrudi AN, Mukhaleva E, Ma N, Koers EJ, Chen Q, Edwards PC, Huang F, Kim J, Mancia F, Veprintsev DB, Vaidehi N, Weyand SN, Tate CG. (2024)
Kofler L, Grundmann L, Gerhalter M, Prattes M, Merl-Pham J, Zisser G, Grishkovskaya I, Hodirnau VV, Vareka M, Breinbauer R, Hauck SM, Haselbach D, Bergler H. (2024)