EMPIAR-11133
Structures of the Cyanobacterial Phycobilisome in the Light-harvesting and Photoprotected States [multiple data sets in MRC and TIFF formats]
Publication:

Structures of a phycobilisome in light-harvesting and photoprotected states

Domínguez-Martín MA, Sauer PV, Kirst H, Sutter M, Bína D, Greber BJ, Nogales E, Polívka T, Kerfeld CA

Nature (2022)

PMID: 36045294

Deposited:
2022-06-30
Released:
2022-09-12
Last modified:
2022-09-12
Imageset size:
16.68 TB
Imageset DOI:
Experimental metadata:
Download xml json
Contains:
  • micrographs - single frame
  • micrographs - multiframe
1. Dataset 3: PBS-OCP micrographs motion corrected and streptavidin subtracted
Category:
micrographs - single frame
Image format:
MRC
No. of images or tilt series:
13427
Image size:
(5760, 4092)
Pixel type:
32 BIT FLOAT
Pixel spacing:
(1.05, 1.05)
Details:
Data collected on 300kV Krios G3 with K3 detector. Micrographs are averaged from 50 frame movies with a total dose of 50e/A2. After motion correction in Relion3.1, streptavidin lattice signal was subtracted using custom Matlab scripts.
Files:
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2. Dataset 2: PBS-apo micrographs motion corrected and streptavidin subtracted
Category:
micrographs - single frame
Image format:
MRC
No. of images or tilt series:
12051
Image size:
(5760, 4092)
Pixel type:
32 BIT FLOAT
Pixel spacing:
(1.05, 1.05)
Details:
Data collected on 300kV Krios G3 with K3 detector. Micrographs are averaged from 50 frame movies with a total dose of 50e/A2. After motion correction in Relion3.1, streptavidin lattice signal was subtracted using custom Matlab scripts.
Files:
Loading...
3. Dataset 1: PBS-apo micrographs motion corrected and streptavidin subtracted
Category:
micrographs - single frame
Image format:
MRC
No. of images or tilt series:
1954
Image size:
(5760, 4092)
Pixel type:
32 BIT FLOAT
Pixel spacing:
(1.115, 1.115)
Details:
Data collected on 200kV Arctica with K3 detector. Micrographs are averaged from 50 frame movies with a total dose of 50e/A2. After motion correction in Relion3.1, streptavidin lattice signal was subtracted using custom Matlab scripts.
Files:
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4. Dataset 3: PBS-OCP raw movies unaligned and unsubtracted
Category:
micrographs - multiframe
Image format:
TIFF
No. of images or tilt series:
13452
Image size:
(11520, 8184)
Pixel type:
UNSIGNED BYTE
Pixel spacing:
(0.524, 0.524)
Details:
Data collected on 300kV Krios G3 with K3 detector. Total dose of 50e/A2. Contains streptavidin signal. Gain reference in .dm4 format.
Files:
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5. Dataset 2: PBS-apo raw movies unaligned and unsubtracted
Category:
micrographs - multiframe
Image format:
TIFF
No. of images or tilt series:
12387
Image size:
(11520, 8184)
Pixel type:
UNSIGNED BYTE
Pixel spacing:
(0.524, 0.524)
Details:
Data collected on 300kV Krios G3 with K3 detector. Total dose of 50e/A2. Contains streptavidin signal. Gain reference in .dm4 format.
Files:
Loading...
Files:
Loading...

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