EMPIAR-11133 - Structures of the Cyanobacterial Phycobilisome in the Light-harvesting and Photoprotected States [multiple data sets in MRC and TIFF formats]

EMPIAR-11133

Structures of the Cyanobacterial Phycobilisome in the Light-harvesting and Photoprotected States [multiple data sets in MRC and TIFF formats]

Publication:

Structures of a phycobilisome in light-harvesting and photoprotected states

Domínguez-Martín MA, Sauer PV, Kirst H, Sutter M, Bína D, Greber BJ, Nogales E, Polívka T, Kerfeld CA

Nature (2022)

PMID: 36045294

DOI: 10.1038/s41586-022-05156-4

Related EMDB entry:

EMD-25070 EMD-25029 EMD-25031 EMD-25033 EMD-25032 EMD-25028 EMD-25030 EMD-25071 EMD-25069 EMD-25068

Deposited:

2022-06-30

Released:

2022-09-12

Last modified:

2022-09-12

Imageset size:

18.34 TB

Imageset DOI:

10.6019/EMPIAR-11133

Experimental metadata:

Download xml json

Contains:

micrographs - single frame
micrographs - multiframe

Image Sets

1. Dataset 3: PBS-OCP micrographs motion corrected and streptavidin subtracted

Category:

micrographs - single frame

Image format:

MRC

No. of images or tilt series:

13427

Image size:

(5760, 4092)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(1.05, 1.05)

Details:

Data collected on 300kV Krios G3 with K3 detector. Micrographs are averaged from 50 frame movies with a total dose of 50e/A2. After motion correction in Relion3.1, streptavidin lattice signal was subtracted using custom Matlab scripts.

Files:

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2. Dataset 2: PBS-apo micrographs motion corrected and streptavidin subtracted

Category:

micrographs - single frame

Image format:

MRC

No. of images or tilt series:

12051

Image size:

(5760, 4092)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(1.05, 1.05)

Details:

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

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3. Dataset 1: PBS-apo micrographs motion corrected and streptavidin subtracted

Category:

micrographs - single frame

Image format:

MRC

No. of images or tilt series:

1954

Image size:

(5760, 4092)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(1.115, 1.115)

Details:

Data collected on 200kV Arctica with K3 detector. Micrographs are averaged from 50 frame movies with a total dose of 50e/A2. After motion correction in Relion3.1, streptavidin lattice signal was subtracted using custom Matlab scripts.

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

4. Dataset 3: PBS-OCP raw movies unaligned and unsubtracted

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

13452

Image size:

(11520, 8184)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(0.524, 0.524)

Details:

Data collected on 300kV Krios G3 with K3 detector. Total dose of 50e/A2. Contains streptavidin signal. Gain reference in .dm4 format.

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

5. Dataset 2: PBS-apo raw movies unaligned and unsubtracted

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

12387

Image size:

(11520, 8184)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(0.524, 0.524)

Details:

Data collected on 300kV Krios G3 with K3 detector. Total dose of 50e/A2. Contains streptavidin signal. Gain reference in .dm4 format.

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

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Arora S, Pan SH, Kumar S, Singh SK, Chauhan U, Alhalabi W, Arya V, Alsulami BS, Hsu CH, Chui KT, Gupta BB. (2025)

PERC: a suite of software tools for the curation of cryoEM data with application to simulation, modeling and machine learning.

Costa-Gomes B, Greer J, Juraschko N, Parkhurst J, Mirecka J, Famili M, Rangel-Smith C, Strickson O, Lowe A, Basham M, Burnley T. (2025)

Self-supervised learning for generalizable particle picking in cryo-EM micrographs.

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Global conformations of <i>Pichia pastoris</i> complex I are distinguished by the binding of a unique interdomain bridging subunit.

Lee CS, Grba DN, Wright JJ, Ivanov BS, Hirst J. (2025)

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