EMPIAR-11075 - Single-particle cryo-EM dataset of the Vairimorpha necatrix ribosome [3284 multi-frame micrographs composed of 32 frames each in TIFF format]

EMPIAR-11075

Single-particle cryo-EM dataset of the Vairimorpha necatrix ribosome [3284 multi-frame micrographs composed of 32 frames each in TIFF format]

Publication:

Evolutionary compaction and adaptation visualized by the structure of the dormant microsporidian ribosome

Barandun J, Hunziker M, Vossbrinck CR, Klinge S

Nature microbiology 4 (2019) 1798-1804

PMID: 31332387

DOI: 10.1038/s41564-019-0514-6

Related EMDB entry:

EMD-4935 EMD-4931 EMD-4932 EMD-4933 EMD-4934

Deposited:

2021-09-03

Released:

2022-07-01

Last modified:

2022-07-01

Imageset size:

1.13 TB

Imageset DOI:

10.6019/EMPIAR-11075

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe

Image Sets

1. Unaligned multi-frame micrographs of the Vairimorpha necatrix ribosome

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

3284

Image size:

(None, None)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(0.6, 0.6)

Details:

Movies with 32 frames were collected at a dose of 8 electrons per pixel per second over an exposure time of 8 seconds. Defocus range of 0.5–4 μm, pixel size of 1.2 Å (0.6 Å super-resolution).

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Citations

High-resolution ab initio reconstruction enables cryo-EM structure determination of small particles

Kim K, Li H, Clarke OB. (2025)

CLEM-Reg: an automated point cloud-based registration algorithm for volume correlative light and electron microscopy.

Krentzel D, Elphick M, Domart MC, Peddie CJ, Laine RF, Shand C, Henriques R, Collinson LM, Jones ML. (2025)

CryoEMNet driven symmetry-aware molecular reconstruction through deep learning enhanced electron microscopy.

Arora S, Pan SH, Kumar S, Singh SK, Chauhan U, Alhalabi W, Arya V, Alsulami BS, Hsu CH, Chui KT, Gupta BB. (2025)

PERC: a suite of software tools for the curation of cryoEM data with application to simulation, modeling and machine learning.

Costa-Gomes B, Greer J, Juraschko N, Parkhurst J, Mirecka J, Famili M, Rangel-Smith C, Strickson O, Lowe A, Basham M, Burnley T. (2025)

Self-supervised learning for generalizable particle picking in cryo-EM micrographs.

Zamanos A, Koromilas P, Bouritsas G, Kastritis PL, Panagakis Y. (2025)

Laser flash melting cryo-EM samples to overcome preferred orientation.

Straub MS, Harder OF, Mowry NJ, Barrass SV, Hruby J, Drabbels M, Lorenz UJ. (2025)

In situ structure determination of conformationally flexible targets using nextPYP.

Liu HF, Zhou Y, Huang Q, Martin J, Bartesaghi A. (2025)

EMICSS: added-value annotations for EMDB entries.

Duraisamy AK, Fonseca N, Kleywegt GJ, Patwardhan A, Morris KL. (2025)

Global conformations of <i>Pichia pastoris</i> complex I are distinguished by the binding of a unique interdomain bridging subunit.

Lee CS, Grba DN, Wright JJ, Ivanov BS, Hirst J. (2025)

Overcoming Preferred Orientation in Cryo-EM With Ultrasonic Excitation During Vitrification

Williams HM, Curtis WA, Haubner M, Hruby J, Drabbels M, Lorenz UJ. (2025)

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