EMPIAR-11055
Crosshair, semi-automated targeting for electron microscopy with a motorised ultramicrotome [multiple data sets in TIFF and PNG formats]
Publication:

Crosshair, semi-automated targeting for electron microscopy with a motorised ultramicrotome

Meechan K, Guan W, Riedinger A, Stankova V, Yoshimura A, Pipitone R, Milberger A, Schaar H, Romero-Brey I, Templin R, Peddie C J, Schieber N L, Jones M L, Collinson L, Schwab Y

BioRxiv (2022)

Related EMDB entry:
Deposited:
2022-05-09
Released:
2022-06-01
Last modified:
2022-06-01
Imageset size:
162.10 GB
Imageset DOI:
Experimental metadata:
Download xml json
Contains:
  • reconstructed volumes
1. sample_a_before_targeting.tif
Category:
reconstructed volumes
Image format:
TIFF
No. of images or tilt series:
1
Image size:
(1000, 660)
Pixel type:
UNSIGNED BYTE
Pixel spacing:
(None, None)
Details:
Micro-CT of sample a before targeting (ready for crosshair)
Files:
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2. sample_a_after_targeting.tif
Category:
reconstructed volumes
Image format:
TIFF
No. of images or tilt series:
1
Image size:
(738, 870)
Pixel type:
UNSIGNED BYTE
Pixel spacing:
(None, None)
Details:
Micro-CT of sample a after targeting (ready for accuracy measures)
Files:
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3. Raw sample a micro-CT - before reconstruction and before targeting
Category:
reconstructed volumes
Image format:
TIFF
No. of images or tilt series:
939
Image size:
(2016, 1344)
Pixel type:
UNSIGNED 16 BIT INTEGER
Pixel spacing:
(None, None)
Details:
Raw sample a micro-CT (before reconstruction and before targeting) from Bruker Skyscan 1272, along with log files of scan settings
Files:
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4. Raw sample a micro-CT - after reconstruction and before targeting
Category:
reconstructed volumes
Image format:
PNG
No. of images or tilt series:
479
Image size:
(2016, 2016)
Pixel type:
UNSIGNED BYTE
Pixel spacing:
(None, None)
Details:
Raw sample a micro-CT (after reconstruction and before targeting) from Bruker Skyscan 1272, along with log files of scan settings
Files:
Loading...
5. Raw sample a micro-CT - before reconstruction and after targeting
Category:
reconstructed volumes
Image format:
TIFF
No. of images or tilt series:
1799
Image size:
(2016, 1344)
Pixel type:
UNSIGNED 16 BIT INTEGER
Pixel spacing:
(None, None)
Details:
Raw sample a micro-CT (before reconstruction and after targeting) from Bruker Skyscan 1272, along with log files of scan settings
Files:
Loading...
6. Raw sample a micro-CT - after reconstruction and after targeting
Category:
reconstructed volumes
Image format:
PNG
No. of images or tilt series:
465
Image size:
(2016, 2016)
Pixel type:
UNSIGNED 16 BIT INTEGER
Pixel spacing:
(None, None)
Details:
Raw sample a micro-CT (after reconstruction and after targeting) from Bruker Skyscan 1272, along with log files of scan settings
Files:
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7. sample_b_before_targeting.tif
Category:
reconstructed volumes
Image format:
TIFF
No. of images or tilt series:
1
Image size:
(1046, 688)
Pixel type:
UNSIGNED BYTE
Pixel spacing:
(None, None)
Details:
Micro-CT of sample b before targeting (ready for crosshair)
Files:
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8. sample_b_after_targeting.tif
Category:
reconstructed volumes
Image format:
TIFF
No. of images or tilt series:
1
Image size:
(1224, 774)
Pixel type:
UNSIGNED BYTE
Pixel spacing:
(None, None)
Details:
Micro-CT of sample b after targeting (ready for accuracy measures)
Files:
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9. Raw sample b micro-CT - before reconstruction and before targeting
Category:
reconstructed volumes
Image format:
TIFF
No. of images or tilt series:
1799
Image size:
(2016, 1344)
Pixel type:
UNSIGNED 16 BIT INTEGER
Pixel spacing:
(None, None)
Details:
Raw sample b micro-CT (before reconstruction and before targeting) from Bruker Skyscan 1272, along with log files of scan settings
Files:
Loading...
10. Raw sample b micro-CT - after reconstruction and before targeting
Category:
reconstructed volumes
Image format:
PNG
No. of images or tilt series:
441
Image size:
(2016, 2016)
Pixel type:
UNSIGNED 16 BIT INTEGER
Pixel spacing:
(None, None)
Details:
Raw sample b micro-CT (after reconstruction and before targeting) from Bruker Skyscan 1272, along with log files of scan settings
Files:
Loading...
11. Raw sample b micro-CT - before reconstruction and after targeting
Category:
reconstructed volumes
Image format:
TIFF
No. of images or tilt series:
1799
Image size:
(2016, 1344)
Pixel type:
UNSIGNED 16 BIT INTEGER
Pixel spacing:
(None, None)
Details:
Raw sample b micro-CT (before reconstruction and after targeting) from Bruker Skyscan 1272, along with log files of scan settings
Files:
Loading...
12. Raw sample b micro-CT - after reconstruction and after targeting
Category:
reconstructed volumes
Image format:
PNG
No. of images or tilt series:
559
Image size:
(2016, 2016)
Pixel type:
UNSIGNED 16 BIT INTEGER
Pixel spacing:
(None, None)
Details:
Raw sample b micro-CT (after reconstruction and after targeting) from Bruker Skyscan 1272, along with log files of scan settings
Files:
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13. sample_c_before_targeting.tif
Category:
reconstructed volumes
Image format:
TIFF
No. of images or tilt series:
1
Image size:
(838, 568)
Pixel type:
UNSIGNED BYTE
Pixel spacing:
(None, None)
Details:
Micro-CT of sample c before targeting (ready for crosshair)
Files:
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14. sample_c_after_targeting.tif
Category:
reconstructed volumes
Image format:
TIFF
No. of images or tilt series:
1
Image size:
(663, 867)
Pixel type:
UNSIGNED BYTE
Pixel spacing:
(None, None)
Details:
Micro-CT of sample c after targeting (ready for accuracy measures)
Files:
Loading...
15. Raw sample c micro-CT - before reconstruction and before targeting
Category:
reconstructed volumes
Image format:
TIFF
No. of images or tilt series:
1799
Image size:
(2016, 1344)
Pixel type:
UNSIGNED 16 BIT INTEGER
Pixel spacing:
(None, None)
Details:
Raw sample c micro-CT (before reconstruction and before targeting) from Bruker Skyscan 1272, along with log files of scan settings
Files:
Loading...
16. Raw sample c micro-CT - after reconstruction and before targeting
Category:
reconstructed volumes
Image format:
PNG
No. of images or tilt series:
462
Image size:
(2016, 2016)
Pixel type:
UNSIGNED 16 BIT INTEGER
Pixel spacing:
(None, None)
Details:
Raw sample c micro-CT (after reconstruction and before targeting) from Bruker Skyscan 1272, along with log files of scan settings
Files:
Loading...
17. Raw sample c micro-CT - before reconstruction and after targeting
Category:
reconstructed volumes
Image format:
TIFF
No. of images or tilt series:
1799
Image size:
(2016, 1344)
Pixel type:
UNSIGNED 16 BIT INTEGER
Pixel spacing:
(None, None)
Details:
Raw sample c micro-CT (before reconstruction and after targeting) from Bruker Skyscan 1272, along with log files of scan settings
Files:
Loading...
18. Raw sample c micro-CT - after reconstruction and after targeting
Category:
reconstructed volumes
Image format:
PNG
No. of images or tilt series:
430
Image size:
(2016, 2016)
Pixel type:
UNSIGNED 16 BIT INTEGER
Pixel spacing:
(None, None)
Details:
Raw sample c micro-CT (after reconstruction and after targeting) from Bruker Skyscan 1272, along with log files of scan settings
Files:
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19. sample_d_before_targeting.tif
Category:
reconstructed volumes
Image format:
TIFF
No. of images or tilt series:
1
Image size:
(894, 576)
Pixel type:
UNSIGNED BYTE
Pixel spacing:
(None, None)
Details:
Micro-CT of sample d before targeting (ready for crosshair)
Files:
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20. sample_d_after_targeting.tif
Category:
reconstructed volumes
Image format:
TIFF
No. of images or tilt series:
1
Image size:
(939, 762)
Pixel type:
UNSIGNED BYTE
Pixel spacing:
(None, None)
Details:
Micro-CT of sample d after targeting (ready for accuracy measures)
Files:
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21. Raw sample d micro-CT - before reconstruction and before targeting
Category:
reconstructed volumes
Image format:
TIFF
No. of images or tilt series:
1799
Image size:
(2016, 1344)
Pixel type:
UNSIGNED 16 BIT INTEGER
Pixel spacing:
(None, None)
Details:
Raw sample d micro-CT (before reconstruction and before targeting) from Bruker Skyscan 1272, along with log files of scan settings
Files:
Loading...
22. Raw sample d micro-CT - after reconstruction and before targeting
Category:
reconstructed volumes
Image format:
PNG
No. of images or tilt series:
519
Image size:
(2016, 2016)
Pixel type:
UNSIGNED 16 BIT INTEGER
Pixel spacing:
(None, None)
Details:
Raw sample d micro-CT (after reconstruction and before targeting) from Bruker Skyscan 1272, along with log files of scan settings
Files:
Loading...
23. Raw sample d micro-CT - before reconstruction and after targeting
Category:
reconstructed volumes
Image format:
TIFF
No. of images or tilt series:
1799
Image size:
(2016, 1344)
Pixel type:
UNSIGNED 16 BIT INTEGER
Pixel spacing:
(None, None)
Details:
Raw sample d micro-CT (before reconstruction and after targeting) from Bruker Skyscan 1272, along with log files of scan settings
Files:
Loading...
24. Raw sample d micro-CT - after reconstruction and after targeting
Category:
reconstructed volumes
Image format:
PNG
No. of images or tilt series:
459
Image size:
(2016, 2016)
Pixel type:
UNSIGNED 16 BIT INTEGER
Pixel spacing:
(None, None)
Details:
Raw sample d micro-CT (after reconstruction and after targeting) from Bruker Skyscan 1272, along with log files of scan settings
Files:
Loading...
25. sample_e_before_targeting.tif
Category:
reconstructed volumes
Image format:
TIFF
No. of images or tilt series:
1
Image size:
(770, 706)
Pixel type:
UNSIGNED BYTE
Pixel spacing:
(None, None)
Details:
Micro-CT of sample e before targeting (ready for crosshair)
Files:
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26. sample_e_after_targeting.tif
Category:
reconstructed volumes
Image format:
TIFF
No. of images or tilt series:
1
Image size:
(807, 846)
Pixel type:
UNSIGNED BYTE
Pixel spacing:
(None, None)
Details:
Micro-CT of sample e after targeting (ready for accuracy measures)
Files:
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27. Raw sample e micro-CT - before reconstruction and before targeting
Category:
reconstructed volumes
Image format:
TIFF
No. of images or tilt series:
939
Image size:
(2016, 1344)
Pixel type:
UNSIGNED 16 BIT INTEGER
Pixel spacing:
(None, None)
Details:
Raw sample e micro-CT (before reconstruction and before targeting) from Bruker Skyscan 1272, along with log files of scan settings
Files:
Loading...
28. Raw sample e micro-CT - after reconstruction and before targeting
Category:
reconstructed volumes
Image format:
PNG
No. of images or tilt series:
463
Image size:
(2016, 2016)
Pixel type:
UNSIGNED 16 BIT INTEGER
Pixel spacing:
(None, None)
Details:
Raw sample e micro-CT (after reconstruction and before targeting) from Bruker Skyscan 1272, along with log files of scan settings
Files:
Loading...
29. Raw sample e micro-CT - before reconstruction and after targeting
Category:
reconstructed volumes
Image format:
TIFF
No. of images or tilt series:
1799
Image size:
(2016, 1344)
Pixel type:
UNSIGNED 16 BIT INTEGER
Pixel spacing:
(None, None)
Details:
Raw sample e micro-CT (before reconstruction and after targeting) from Bruker Skyscan 1272, along with log files of scan settings
Files:
Loading...
30. Raw sample e micro-CT - after reconstruction and after targeting
Category:
reconstructed volumes
Image format:
PNG
No. of images or tilt series:
443
Image size:
(2016, 2016)
Pixel type:
UNSIGNED 16 BIT INTEGER
Pixel spacing:
(None, None)
Details:
Raw sample e micro-CT (after reconstruction and after targeting) from Bruker Skyscan 1272, along with log files of scan settings
Files:
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31. sample_f_before_targeting.tif
Category:
reconstructed volumes
Image format:
TIFF
No. of images or tilt series:
1
Image size:
(994, 1015)
Pixel type:
UNSIGNED BYTE
Pixel spacing:
(None, None)
Details:
Micro-CT of sample f before targeting (ready for crosshair)
Files:
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32. sample_f_after_targeting.tif
Category:
reconstructed volumes
Image format:
TIFF
No. of images or tilt series:
1
Image size:
(993, 1015)
Pixel type:
UNSIGNED BYTE
Pixel spacing:
(None, None)
Details:
Micro-CT of sample f after targeting (ready for accuracy measures)
Files:
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33. Raw sample f micro-CT - after reconstruction and before targeting
Category:
reconstructed volumes
Image format:
TIFF
No. of images or tilt series:
1
Image size:
(994, 1015)
Pixel type:
UNSIGNED 16 BIT INTEGER
Pixel spacing:
(None, None)
Details:
Raw sample f micro-CT (after reconstruction and before targeting) from Versa 510 X-ray microscope (Zeiss). The corresponding before reconstruction file (.txrm) is available in the same directory.
Files:
Loading...
34. Raw sample f micro-CT - after reconstruction and after targeting
Category:
reconstructed volumes
Image format:
TIFF
No. of images or tilt series:
1
Image size:
(993, 1015)
Pixel type:
UNSIGNED 16 BIT INTEGER
Pixel spacing:
(None, None)
Details:
Raw sample f micro-CT (after reconstruction and after targeting) from Versa 510 X-ray microscope (Zeiss). The corresponding before reconstruction file (.txrm) is available in the same directory.
Files:
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35. sample_g_before_targeting.tif
Category:
reconstructed volumes
Image format:
TIFF
No. of images or tilt series:
1
Image size:
(992, 1015)
Pixel type:
UNSIGNED BYTE
Pixel spacing:
(None, None)
Details:
Micro-CT of sample g before targeting (ready for crosshair)
Files:
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36. sample_g_after_targeting.tif
Category:
reconstructed volumes
Image format:
TIFF
No. of images or tilt series:
1
Image size:
(1015, 987)
Pixel type:
UNSIGNED BYTE
Pixel spacing:
(None, None)
Details:
Micro-CT of sample g after targeting (ready for accuracy measures)
Files:
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37. Raw sample g micro-CT - after reconstruction and before targeting
Category:
reconstructed volumes
Image format:
TIFF
No. of images or tilt series:
1
Image size:
(992, 1015)
Pixel type:
UNSIGNED 16 BIT INTEGER
Pixel spacing:
(None, None)
Details:
Raw sample g micro-CT (after reconstruction and before targeting) from Versa 510 X-ray microscope (Zeiss). The corresponding before reconstruction file (.txrm) is available in the same directory.
Files:
Loading...
38. Raw sample g micro-CT - after reconstruction and after targeting
Category:
reconstructed volumes
Image format:
TIFF
No. of images or tilt series:
1
Image size:
(987, 1015)
Pixel type:
UNSIGNED 16 BIT INTEGER
Pixel spacing:
(None, None)
Details:
Raw sample g micro-CT (after reconstruction and after targeting) from Versa 510 X-ray microscope (Zeiss). The corresponding before reconstruction file (.txrm) is available in the same directory.
Files:
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39. sample_h_before_targeting.tif
Category:
reconstructed volumes
Image format:
TIFF
No. of images or tilt series:
1
Image size:
(992, 1015)
Pixel type:
UNSIGNED BYTE
Pixel spacing:
(None, None)
Details:
Micro-CT of sample h before targeting (ready for crosshair)
Files:
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40. sample_h_after_targeting.tif
Category:
reconstructed volumes
Image format:
TIFF
No. of images or tilt series:
1
Image size:
(991, 1015)
Pixel type:
UNSIGNED BYTE
Pixel spacing:
(None, None)
Details:
Micro-CT of sample h after targeting (ready for accuracy measures)
Files:
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41. Raw sample h micro-CT - after reconstruction and before targeting
Category:
reconstructed volumes
Image format:
TIFF
No. of images or tilt series:
1
Image size:
(992, 1015)
Pixel type:
UNSIGNED 16 BIT INTEGER
Pixel spacing:
(None, None)
Details:
Raw sample h micro-CT (after reconstruction and before targeting) from Versa 510 X-ray microscope (Zeiss). The corresponding before reconstruction file (.txrm) is available in the same directory.
Files:
Loading...
42. Raw sample h micro-CT - after reconstruction and after targeting
Category:
reconstructed volumes
Image format:
TIFF
No. of images or tilt series:
1
Image size:
(991, 1015)
Pixel type:
UNSIGNED 16 BIT INTEGER
Pixel spacing:
(None, None)
Details:
Raw sample h micro-CT (after reconstruction and after targeting) from Versa 510 X-ray microscope (Zeiss). The corresponding before reconstruction file (.txrm) is available in the same directory.
Files:
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43. sample_i_before_targeting.tif
Category:
reconstructed volumes
Image format:
TIFF
No. of images or tilt series:
1
Image size:
(992, 1015)
Pixel type:
UNSIGNED BYTE
Pixel spacing:
(None, None)
Details:
Micro-CT of sample i before targeting (ready for crosshair)
Files:
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44. sample_i_after_targeting.tif
Category:
reconstructed volumes
Image format:
TIFF
No. of images or tilt series:
1
Image size:
(1015, 991)
Pixel type:
UNSIGNED BYTE
Pixel spacing:
(None, None)
Details:
Micro-CT of sample i after targeting (ready for accuracy measures)
Files:
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45. Raw sample i micro-CT - after reconstruction and before targeting
Category:
reconstructed volumes
Image format:
TIFF
No. of images or tilt series:
1
Image size:
(992, 1015)
Pixel type:
UNSIGNED 16 BIT INTEGER
Pixel spacing:
(None, None)
Details:
Raw sample i micro-CT (after reconstruction and before targeting) from Versa 510 X-ray microscope (Zeiss). The corresponding before reconstruction file (.txrm) is available in the same directory.
Files:
Loading...
46. Raw sample i micro-CT - after reconstruction and after targeting
Category:
reconstructed volumes
Image format:
TIFF
No. of images or tilt series:
1
Image size:
(991, 1015)
Pixel type:
UNSIGNED 16 BIT INTEGER
Pixel spacing:
(None, None)
Details:
Raw sample i micro-CT (after reconstruction and after targeting) from Versa 510 X-ray microscope (Zeiss). The corresponding before reconstruction file (.txrm) is available in the same directory.
Files:
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47. sample_j_before_targeting.tif
Category:
reconstructed volumes
Image format:
TIFF
No. of images or tilt series:
1
Image size:
(993, 1015)
Pixel type:
UNSIGNED BYTE
Pixel spacing:
(None, None)
Details:
Micro-CT of sample j before targeting (ready for crosshair)
Files:
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48. sample_j_after_targeting.tif
Category:
reconstructed volumes
Image format:
TIFF
No. of images or tilt series:
1
Image size:
(1015, 986)
Pixel type:
UNSIGNED BYTE
Pixel spacing:
(None, None)
Details:
Micro-CT of sample j after targeting (ready for accuracy measures)
Files:
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49. Raw sample j micro-CT - after reconstruction and before targeting
Category:
reconstructed volumes
Image format:
TIFF
No. of images or tilt series:
1
Image size:
(993, 1015)
Pixel type:
UNSIGNED 16 BIT INTEGER
Pixel spacing:
(None, None)
Details:
Raw sample j micro-CT (after reconstruction and before targeting) from Versa 510 X-ray microscope (Zeiss). The corresponding before reconstruction file (.txrm) is available in the same directory.
Files:
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50. Raw sample j micro-CT - after reconstruction and after targeting
Category:
reconstructed volumes
Image format:
TIFF
No. of images or tilt series:
1
Image size:
(986, 1015)
Pixel type:
UNSIGNED 16 BIT INTEGER
Pixel spacing:
(None, None)
Details:
Raw sample j micro-CT (after reconstruction and after targeting) from Versa 510 X-ray microscope (Zeiss). The corresponding before reconstruction file (.txrm) is available in the same directory.
Files:
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