EMPIAR-11043
Architecture of the human erythrocyte ankyrin-1 complex [multiple data sets in TIFF, MRC and MRCS formats]
Publication:

Architecture of the human erythrocyte ankyrin-1 complex

Vallese F, Kim K, Yen LY, Johnston JD, Noble AJ, Calì T, Clarke OB

Nature structural & molecular biology 29 (2022) 706-718

PMID: 35835865

Related EMDB entry:
Deposited:
2022-02-08
Released:
2022-08-08
Last modified:
2022-09-09
Imageset size:
5.24 TB
Imageset DOI:
Experimental metadata:
Download xml json
Contains:
  • micrographs - multiframe
  • micrographs - single frame
  • picked particles - single frame - unprocessed
1. Unaligned multi-frame movies of ankyrin-1 complex
Category:
micrographs - multiframe
Image format:
TIFF
No. of images or tilt series:
14926
Image size:
(5760, 4092)
Pixel type:
UNSIGNED BYTE
Pixel spacing:
(0.83, 0.83)
Details:
The micrographs are of 50 raw movie frames accumulating 58.2 electrons per squared Angstrom in a 2.5 second exposure.
Particle positions are in .star files with X and Y coordinates in 3rd and 4th column respectively.

consensus_particles_stack.mrcs contains the particle images of the ankyrin-1 complex consensus particle set.

Band3_GPA_particles.star contains the particle coordinates and CTF parameters of the band3-GPA partcle set.
Files:
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2. Aligned micrographs of ankyrin-1 complex
Category:
micrographs - single frame
Image format:
MRC
No. of images or tilt series:
14926
Image size:
(5760, 4092)
Pixel type:
UNSIGNED BYTE
Pixel spacing:
(0.83, 0.83)
Details:
The raw movie frames are aligned with patch motion correction in cryoSPARC. (50 frames, total dose: 58.2e-/A2, Cs=2.7)
Particle positions are in .star files with X and Y coordinates in 3rd and 4th column respectively.
consensus_particles_stack.mrcs contains the particle images of the ankyrin-1 complex consensus particle set.
Band3_GPA_particles.star contains the particle coordinates and CTF parameters of the band3-GPA partcle set.
Files:
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3. Consensus particle stack for the ankyrin-1 complex
Category:
picked particles - single frame - unprocessed
Image format:
MRCS
No. of images or tilt series:
710437
Image size:
(450, 450)
Pixel type:
UNSIGNED BYTE
Pixel spacing:
(0.83, 0.83)
Details:
710437 particle image stack is saved as consensus_particles_stack.mrcs

Particle coordinates and CTF parameters are saved in consensus_particles_stack.star

Pixel size: 0.83 Å/pix
Box size: 450 pix
Files:
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