EMPIAR-11000 - Cryo-EM structure of human U2 snRNP after ATP-dependent remodeling [multiple data sets in TIFF and MRCS formats]

EMPIAR-11000

Cryo-EM structure of human U2 snRNP after ATP-dependent remodeling [multiple data sets in TIFF and MRCS formats]

Publication:

Structural basis of branch site recognition by the human spliceosome

Tholen J, Razew M, Weis F, Galej WP

Science (New York, N.Y.) 375 (2021) 50-57

PMID: 34822310

DOI: 10.1126/science.abm4245

Related EMDB entry:

EMD-13812

Deposited:

2022-03-29

Released:

2022-04-22

Last modified:

2022-04-22

Imageset size:

1.45 TB

Imageset DOI:

10.6019/EMPIAR-11000

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe
picked particles - single frame - processed

Image Sets

1. Unaligned multi-frame micrographs of the U2 snRNP after ATP-dependent remodeling

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

7809

Image size:

(5760, 4092)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(0.64, 0.64)

Details:

Unaligned movies (40 frames) acquired over 1 s with a total 50.76e/Å2 dose with defocus range of -0.8 to -1.8 μm in 0.1 μm steps. Gain reference is CountRef_114-1_001.mrc

Files:

Archives and downloads the selected files into an uncompressed zip file.

Depending on the number and size of files to be downloaded, this can take an enormous amount of time. Also, this feature is unstable and may not work properly, and checksums of downloaded files are not verified.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

2. Final polished particles of the U2 snRNP after ATP-dependent remodeling

Category:

picked particles - single frame - processed

Image format:

MRCS

No. of images or tilt series:

7611

Image size:

(640, 640)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(0.64, 0.64)

Details:

Polished particles after auto-refinement in Relion3.1. In the star file the columns rlnMicrographName and rlnImageName are not correct: The paths have to be changed to point to the mrc/mrcs files. The final resolution was 2.15 A. The 3' domain of U2 snRNP could not be resolved.

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

Browse All Files

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

Quick Links

Talks and Tutorials

EMPIAR Quick tour

Statistics

Publications

Re-use case study

EMPIAR in the news

Claim entries to your ORCiD

COVID-19 Data Portal

EMDB

PDBe

BioImage Archive

EMNavigator@PDBj

PDBj

RCSB@PDBj

EMDataResoruce@RCSB,EMDB,NCMI

Citations

Structural basis for HIV-1 antagonism of host APOBEC3G via Cullin E3 ligase.

Ito F, Alvarez-Cabrera AL, Liu S, Yang H, Shiriaeva A, Zhou ZH, Chen XS. (2023)

The 2023 Nucleic Acids Research Database Issue and the online molecular biology database collection.

Rigden DJ, Fernández XM. (2023)

EMPIAR: the Electron Microscopy Public Image Archive.

Iudin A, Korir PK, Somasundharam S, Weyand S, Cattavitello C, Fonseca N, Salih O, Kleywegt GJ, Patwardhan A. (2023)

CLEMSite, a software for automated phenotypic screens using light microscopy and FIB-SEM.

Serra Lleti JM, Steyer AM, Schieber NL, Neumann B, Tischer C, Hilsenstein V, Holtstrom M, Unrau D, Kirmse R, Lucocq JM, Pepperkok R, Schwab Y. (2023)

Structure of a RecT/Redβ family recombinase in complex with a duplex intermediate of DNA annealing.

Caldwell BJ, Norris AS, Karbowski CF, Wiegand AM, Wysocki VH, Bell CE. (2022)

See all citations

Protein Data Bank Japan News

[2024-05-01]

288 new PDB entries have been released on 2024-05-01.

[2024-04-09]

[wwPDB] CASP16 Call for Targets

[2024-04-02]

Announcement of the PDBj Newsletter Vol.24 release

[2024-03-21]

[wwPDB] Paper Published on NMR Restraint Validation

[2024-03-13]

[wwPDB] Paper Published on CryoEM Archiving and Validation Recommendations

[2024-03-06]

[wwPDB] Poster Prize Awarded at The Biophysical Society Meeting

[2024-02-15]

Notice of Service Suspension (ASH, DASH, SeSAW, MAFFTash, Spanner, SFAS)

[2024-02-13]

[wwPDB] Biocurator Milestone: >10,000 Depositions Processed

[2024-02-06]

[wwPDB] Preprint Published on NMR Restraint Validation

[2024-02-05]

[wwPDB] Preprint Published on CryoEM Archiving and Validation Recommendations

See all news