EMPIAR-11000 - Cryo-EM structure of human U2 snRNP after ATP-dependent remodeling [multiple data sets in TIFF and MRCS formats]

EMPIAR-11000

Cryo-EM structure of human U2 snRNP after ATP-dependent remodeling [multiple data sets in TIFF and MRCS formats]

Publication:

Structural basis of branch site recognition by the human spliceosome

Tholen J, Razew M, Weis F, Galej WP

Science (New York, N.Y.) 375 (2021) 50-57

PMID: 34822310

DOI: 10.1126/science.abm4245

Related EMDB entry:

EMD-13812

Deposited:

2022-03-29

Released:

2022-04-22

Last modified:

2022-04-22

Imageset size:

1.45 TB

Imageset DOI:

10.6019/EMPIAR-11000

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe
picked particles - single frame - processed

Image Sets

1. Unaligned multi-frame micrographs of the U2 snRNP after ATP-dependent remodeling

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

7809

Image size:

(5760, 4092)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(0.64, 0.64)

Details:

Unaligned movies (40 frames) acquired over 1 s with a total 50.76e/Å2 dose with defocus range of -0.8 to -1.8 μm in 0.1 μm steps. Gain reference is CountRef_114-1_001.mrc

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2. Final polished particles of the U2 snRNP after ATP-dependent remodeling

Category:

picked particles - single frame - processed

Image format:

MRCS

No. of images or tilt series:

7611

Image size:

(640, 640)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(0.64, 0.64)

Details:

Polished particles after auto-refinement in Relion3.1. In the star file the columns rlnMicrographName and rlnImageName are not correct: The paths have to be changed to point to the mrc/mrcs files. The final resolution was 2.15 A. The 3' domain of U2 snRNP could not be resolved.

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

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