EMPIAR-10991 - Single particle cryo-EM dataset of mitochondrial respiratory complex I from Bos taurus in DDM in the presence of IM1761092 [17203 multi-frame micrographs composed of 40 frames each in TIFF format]

EMPIAR-10991

Single particle cryo-EM dataset of mitochondrial respiratory complex I from Bos taurus in DDM in the presence of IM1761092 [17203 multi-frame micrographs composed of 40 frames each in TIFF format]

Publication:

Structural basis of mammalian respiratory complex I inhibition by medicinal biguanides

Bridges HR, Blaza JN, Yin Z, Chung I, Pollak MN, Hirst J

Science (New York, N.Y.) 379 (2023) 351-357

PMID: 36701435

DOI: 10.1126/science.ade3332

Related EMDB entry:

EMD-14302 EMD-14287 EMD-14282 EMD-14277 EMD-14251 EMD-14297 EMD-14256 EMD-14292 EMD-14307 EMD-14266 EMD-14262 EMD-14267 EMD-14264 EMD-14265 EMD-14269 EMD-14260 EMD-14259 EMD-14268 EMD-14263 EMD-14308 EMD-14309 EMD-14305 EMD-14310 EMD-14306 EMD-14311 EMD-14283 EMD-14288 EMD-14293 EMD-14285 EMD-14290 EMD-14286 EMD-14291 EMD-14284 EMD-14289 EMD-14278 EMD-14273 EMD-14280 EMD-14281 EMD-14275 EMD-14276 EMD-14274 EMD-14279 EMD-14270 EMD-14257 EMD-14252 EMD-14258 EMD-14254 EMD-14255 EMD-14253 EMD-14298 EMD-14303 EMD-14300 EMD-14295 EMD-14296 EMD-14294 EMD-14304 EMD-14301 EMD-14299 EMD-14261 EMD-14272

Deposited:

2022-03-22

Released:

2023-02-28

Last modified:

2023-03-01

Imageset size:

7.19 TB

Imageset DOI:

10.6019/EMPIAR-10991

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe

Image Sets

1. Single particle cryo-EM dataset of mitochondrial respiratory complex I from Bos taurus in DDM in the presence of IM1761092

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

17203

Image size:

(11520, 8184)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(0.536, 0.536)

Details:

Non-gain corrected micrographs were imaged using a Gatan K3 in super-resolution mode and with a GIF BioQuantum energy filter (20 eV slit width) on an FEI 300 keV Titan Krios microscope with a 100 μm objective aperture. 1.7s exposures captured in 40 frames and a total dose of 40 electrons Å-2. Dataset also contains xml files for each image, and gain reference files.

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Citations

High-resolution ab initio reconstruction enables cryo-EM structure determination of small particles

Kim K, Li H, Clarke OB. (2025)

CLEM-Reg: an automated point cloud-based registration algorithm for volume correlative light and electron microscopy.

Krentzel D, Elphick M, Domart MC, Peddie CJ, Laine RF, Shand C, Henriques R, Collinson LM, Jones ML. (2025)

CryoEMNet driven symmetry-aware molecular reconstruction through deep learning enhanced electron microscopy.

Arora S, Pan SH, Kumar S, Singh SK, Chauhan U, Alhalabi W, Arya V, Alsulami BS, Hsu CH, Chui KT, Gupta BB. (2025)

PERC: a suite of software tools for the curation of cryoEM data with application to simulation, modeling and machine learning.

Costa-Gomes B, Greer J, Juraschko N, Parkhurst J, Mirecka J, Famili M, Rangel-Smith C, Strickson O, Lowe A, Basham M, Burnley T. (2025)

Self-supervised learning for generalizable particle picking in cryo-EM micrographs.

Zamanos A, Koromilas P, Bouritsas G, Kastritis PL, Panagakis Y. (2025)

Laser flash melting cryo-EM samples to overcome preferred orientation.

Straub MS, Harder OF, Mowry NJ, Barrass SV, Hruby J, Drabbels M, Lorenz UJ. (2025)

In situ structure determination of conformationally flexible targets using nextPYP.

Liu HF, Zhou Y, Huang Q, Martin J, Bartesaghi A. (2025)

EMICSS: added-value annotations for EMDB entries.

Duraisamy AK, Fonseca N, Kleywegt GJ, Patwardhan A, Morris KL. (2025)

Global conformations of <i>Pichia pastoris</i> complex I are distinguished by the binding of a unique interdomain bridging subunit.

Lee CS, Grba DN, Wright JJ, Ivanov BS, Hirst J. (2025)

Overcoming Preferred Orientation in Cryo-EM With Ultrasonic Excitation During Vitrification

Williams HM, Curtis WA, Haubner M, Hruby J, Drabbels M, Lorenz UJ. (2025)

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