EMPIAR-10986 - Parallel cryo electron tomography (PACE-tomo) of 70S ribosomes (200 kV, side-entry holder) [multiple data sets in MRC format]

EMPIAR-10986

Parallel cryo electron tomography (PACE-tomo) of 70S ribosomes (200 kV, side-entry holder) [multiple data sets in MRC format]

Publication:

Parallel cryo electron tomography on in situ lamellae

Eisenstein F, Yanagisawa H, Kashihara H, Kikkawa M, Tsukita S, Danev R

bioRxiv (2022)

DOI: 10.1101/2022.04.07.487557

Related EMDB entry:

EMD-33116 EMD-33117

Deposited:

2022-03-25

Released:

2022-05-20

Last modified:

2023-01-09

Imageset size:

222.84 GB

Imageset DOI:

10.6019/EMPIAR-10986

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe
tilt series

Image Sets

1. Movie frames for all tilt series gain corrected with outdated gain reference

Category:

micrographs - multiframe

Image format:

MRC

No. of images or tilt series:

525

Image size:

(3710, 3838)

Pixel type:

UNSIGNED 16 BIT INTEGER

Pixel spacing:

(2.16, 2.16)

Details:

applied gain reference was outdated, additional gain reference was created from all frames using relion_estimate_gain and clamping extreme values

Files:

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Depending on the number and size of files to be downloaded, this can take an enormous amount of time. Also, this feature is unstable and may not work properly, and checksums of downloaded files are not verified.

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rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

2. Tilt series and meta data

Category:

tilt series

Image format:

MRC

No. of images or tilt series:

Image size:

(3710, 3838)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(2.16, 2.16)

Details:

Tilt series sorted by tilt angle, mdoc files and dose files usable in IMOD

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

Browse All Files

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

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