EMPIAR-10943 - Tutorial for high-throughput cryo-EM structure determination of amyloids: Dataset 2 [4000 multi-frame micrographs composed of 34 frames each in TIFF format]

EMPIAR-10943

Tutorial for high-throughput cryo-EM structure determination of amyloids: Dataset 2 [4000 multi-frame micrographs composed of 34 frames each in TIFF format]

Publication:

Assembly of recombinant tau into filaments identical to those of Alzheimer's disease and chronic traumatic encephalopathy

Lövestam S, Koh FA, van Knippenberg B, Kotecha A, Murzin AG, Goedert M, Scheres SHW

eLife 11 (2022)

PMID: 35244536

DOI: 10.7554/elife.76494

Related EMDB entry:

EMD-14038

Deposited:

2022-02-03

Released:

2022-03-08

Last modified:

2022-03-28

Imageset size:

1.53 TB

Imageset DOI:

10.6019/EMPIAR-10943

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe

Image Sets

1. Unaligned multi-frame movies in Tiff: Dataset 2

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

4000

Image size:

(None, None)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(0.824, 0.824)

Details:

Movies were collected in EER format and converted into tiff using relion_convert_to_tiff grouping frames by 34 giving a dose of 0.98 e/Å^2. Please see additional files for complete processing pipeline.

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Browse All Files

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

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Citations

Structural basis for HIV-1 antagonism of host APOBEC3G via Cullin E3 ligase.

Ito F, Alvarez-Cabrera AL, Liu S, Yang H, Shiriaeva A, Zhou ZH, Chen XS. (2023)

The 2023 Nucleic Acids Research Database Issue and the online molecular biology database collection.

Rigden DJ, Fernández XM. (2023)

EMPIAR: the Electron Microscopy Public Image Archive.

Iudin A, Korir PK, Somasundharam S, Weyand S, Cattavitello C, Fonseca N, Salih O, Kleywegt GJ, Patwardhan A. (2023)

CLEMSite, a software for automated phenotypic screens using light microscopy and FIB-SEM.

Serra Lleti JM, Steyer AM, Schieber NL, Neumann B, Tischer C, Hilsenstein V, Holtstrom M, Unrau D, Kirmse R, Lucocq JM, Pepperkok R, Schwab Y. (2023)

Structure of a RecT/Redβ family recombinase in complex with a duplex intermediate of DNA annealing.

Caldwell BJ, Norris AS, Karbowski CF, Wiegand AM, Wysocki VH, Bell CE. (2022)

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