EMPIAR-10934 - Cryo-EM multiframe micrographs of human Pol kappa complexed with DNA and monoubiquitylated PCNA [3889 multi-frame micrographs composed of 45 frames each in TIFF format]

EMPIAR-10934

Cryo-EM multiframe micrographs of human Pol kappa complexed with DNA and monoubiquitylated PCNA [3889 multi-frame micrographs composed of 45 frames each in TIFF format]

Publication:

Cryo-EM structure of human Pol κ bound to DNA and mono-ubiquitylated PCNA

Lancey C, Tehseen M, Bakshi S, Percival M, Takahashi M, Sobhy MA, Raducanu VS, Blair K, Muskett FW, Ragan TJ, Crehuet R, Hamdan SM, De Biasio A

Nature communications 12 (2021)

PMID: 34667155

DOI: 10.1038/s41467-021-26251-6

Related EMDB entry:

EMD-12602

Deposited:

2021-12-31

Released:

2022-02-07

Last modified:

2022-02-07

Imageset size:

1.94 TB

Imageset DOI:

10.6019/EMPIAR-10934

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe

Image Sets

1. Multiframe micrographs of human Pol κ complexed with monoubiquitylated PCNA and DNA

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

3889

Image size:

(11520, 8184)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(0.543, 0.543)

Details:

The given pixel size accounts for super resolution binning 1 - the physical pixel size was 1.086 Å/pix. Movies are non-gain normalised - gain reference included.

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Citations

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An unattended image-processing pipeline for on-the-fly quality assessment and 3D exploration in cryo-EM.

Marchán Torres D, Conesa P, Garcia A, Iceta M, Broche L, Gragera M, Linares R, Kwong HS, Chichón FJ, Svensson O, Arranz R, Effantin G, Kandiah E, Carazo JM, Sorzano COS. (2026)

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prismPYP: Power-spectrum and image domain learning for self-supervised micrograph evaluation.

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CryoSIP: unleashing protein high-resolution Cryo-EM via semantic-instance collaborative picking.

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Unbend, correction of local beam-induced sample motion in cryo-EM images using a 3D spline model.

Kong L, Zottig X, Elferich J, Grigorieff N. (2026)

CryoVirusDB: An Annotated Dataset for AI-Based Virus Particle Identification in Cryo-EM Micrographs.

Gyawali R, Dhakal A, Wang L, Cheng J. (2026)

Chart builder: an interactive tool for user driven data visualization in the electron microscopy data bank.

Fonseca N, Duraisamy AK, Wang Z, Somasundharam S, Tayebinia M, de Oliveira LC, Ma M, Turner J, Patwardhan A, Kleywegt GJ, Hartley M, Morris KL. (2026)

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