EMPIAR-10913 - Cryo-EM micrographs of GABA(A)Rs purified from cells expressing human full-length alpha4, beta3 and delta subunits, in presence of HEPES and nanobody Nb25 [18161 multi-frame micrographs composed of 32 frames each in TIFF format]

EMPIAR-10913

Cryo-EM micrographs of GABA(A)Rs purified from cells expressing human full-length alpha4, beta3 and delta subunits, in presence of HEPES and nanobody Nb25 [18161 multi-frame micrographs composed of 32 frames each in TIFF format]

Publication:

Differential assembly diversifies GABA_A receptor structures and signalling

Chirgadze DY, Sente A, Miller KW, Desai R, Aricescu AR, Naydenova K, Malinauskas T, Jounaidi Y, Miehling J, Zhou X, Masiulis S, Hardwick SW

Nature (2022)

PMID: 35355020

DOI: 10.1038/s41586-022-04517-3

Related EMDB entry:

EMD-14068 EMD-14067

Deposited:

2021-12-29

Released:

2022-04-04

Last modified:

2022-04-04

Imageset size:

4.15 TB

Imageset DOI:

10.6019/EMPIAR-10913

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe

Image Sets

1. Unaligned multi-frame micrographs of GABA(A) receptors purified from cells expressing alpha4, beta3 and delta subunits, in presence of HEPES and nanobody Nb25

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

18161

Image size:

(11520, 8184)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(0.325, 0.325)

Details:

K3 super-resolution movies with a total dose of 40 e/A2 and exposure time of 1.31 s.
The gain reference used for data processing is given in data/superres_gain_from_epu.mrc. In RELION:
Gain rotaion: 180 degrees (2)
Gain flip: flip left to right (2)

Files:

Archives and downloads the selected files into an uncompressed zip file.

Depending on the number and size of files to be downloaded, this can take an enormous amount of time. Also, this feature is unstable and may not work properly, and checksums of downloaded files are not verified.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

Browse All Files

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

Quick Links

Talks and Tutorials

EMPIAR Quick tour

Statistics

Publications

Re-use case study

EMPIAR in the news

Claim entries to your ORCiD

COVID-19 Data Portal

EMDB

PDBe

BioImage Archive

EMNavigator@PDBj

PDBj

RCSB@PDBj

EMDataResoruce@RCSB,EMDB,NCMI

Citations

Structural basis for HIV-1 antagonism of host APOBEC3G via Cullin E3 ligase.

Ito F, Alvarez-Cabrera AL, Liu S, Yang H, Shiriaeva A, Zhou ZH, Chen XS. (2023)

The 2023 Nucleic Acids Research Database Issue and the online molecular biology database collection.

Rigden DJ, Fernández XM. (2023)

EMPIAR: the Electron Microscopy Public Image Archive.

Iudin A, Korir PK, Somasundharam S, Weyand S, Cattavitello C, Fonseca N, Salih O, Kleywegt GJ, Patwardhan A. (2023)

CLEMSite, a software for automated phenotypic screens using light microscopy and FIB-SEM.

Serra Lleti JM, Steyer AM, Schieber NL, Neumann B, Tischer C, Hilsenstein V, Holtstrom M, Unrau D, Kirmse R, Lucocq JM, Pepperkok R, Schwab Y. (2023)

Structure of a RecT/Redβ family recombinase in complex with a duplex intermediate of DNA annealing.

Caldwell BJ, Norris AS, Karbowski CF, Wiegand AM, Wysocki VH, Bell CE. (2022)

See all citations

Protein Data Bank Japan News

[2024-05-01]

288 new PDB entries have been released on 2024-05-01.

[2024-04-09]

[wwPDB] CASP16 Call for Targets

[2024-04-02]

Announcement of the PDBj Newsletter Vol.24 release

[2024-03-21]

[wwPDB] Paper Published on NMR Restraint Validation

[2024-03-13]

[wwPDB] Paper Published on CryoEM Archiving and Validation Recommendations

[2024-03-06]

[wwPDB] Poster Prize Awarded at The Biophysical Society Meeting

[2024-02-15]

Notice of Service Suspension (ASH, DASH, SeSAW, MAFFTash, Spanner, SFAS)

[2024-02-13]

[wwPDB] Biocurator Milestone: >10,000 Depositions Processed

[2024-02-06]

[wwPDB] Preprint Published on NMR Restraint Validation

[2024-02-05]

[wwPDB] Preprint Published on CryoEM Archiving and Validation Recommendations

See all news