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EMPIAR-10875
Cryo-EM structure of pioneer factor Cbf1 bound to the nucleosome [6339 multi-frame micrographs composed of 51 frames each in TIFF format]
Publication:

Basic helix-loop-helix pioneer factors interact with the histone octamer to invade nucleosomes and generate nucleosome-depleted regions

Donovan BT, Chen H, Eek P, Meng Z, Jipa C, Tan S, Bai L, Poirier MG

Molecular cell 83 (2023) 1251-1263.e6

PMID: 36996811

Related EMDB entry:
EMD-25406
Deposited:
2021-11-10
Released:
2023-05-23
Last modified:
2023-05-23
Imageset size:
4.09 TB
Imageset DOI:
10.6019/EMPIAR-10875
Experimental metadata:
Download xml
Contains:
  • micrographs - multiframe
Image Sets
1. Unaligned multi-frame micrographs of Cbf1/nucleosome complex
Category:
micrographs - multiframe
Image format:
TIFF
No. of images or tilt series:
6339
Image size:
(11520, 8184)
Pixel type:
UNSIGNED BYTE
Pixel spacing:
(0.644, 0.644)
Details:
Voltage 300 keV, Cs 2.7 mm, total dose 50 e-/Å^2, super-resolution pixel size 0.644 Å/px. The TIFF movies are not gain-corrected, the gain-reference image for the dataset is SuperRef_stack2_00000_X-1Y-1-0 provided in DM4 and MRC formats.
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